Analysis of M phase-regulation by GdX

我们发现细胞周期蛋白F与GdX共价结合（GdX化）。我们的数据表明，GdX化的细胞周期蛋白F调节其蛋白质的稳定性和细胞周期的进展。虽然我们通过生物化学技术鉴定了11个GdX结合蛋白和23个RNF（GdX连接酶的候选者）结合蛋白，但无法鉴定GdX化的E1、E2和E3酶。因此，我们将在未来的发展RNF的功能分析。

We found that cyclin F was covalently conjugated with GdX (GdXylation). Our data suggested that GdXylation of cyclin F regulated its protein stability and cell cycle progression. Although we identified 11 GdX- and 23 RNF (candidate of GdXligase)-binding proteins by biochemical techniques, identification of E1, E2 and E3 enzymes for GdXylation could not be achieved.It was suggested that RNF is functional as a ubiquitinligase in mouse spermatogenesis by analysis of RNF-deficient mice. So we will develop the functional analysis of RNF in future.