Analysis of RNA-binding proteins that regulate meiotic cell cycle in fission yeast

调节裂殖酵母减数分裂细胞周期的 RNA 结合蛋白分析

• 批准号: 20570178
• 负责人: YAMASHITA Akira
• 金额: $ 3.08万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20570178/
• 关键词: 細胞周期; 減数分裂; 発現制御; RNA結合タンパク; 分裂酵母; 分子生物学; RNA結合タンパク質

The aim of this study is to elucidate the function of RNA-binding proteins, Mei2p and Mmi1p, that regulate meiotic cell cycle in fission yeast. It turned out that phosphorylation of RNA polymerase II is crucial for the expression of Mei2p. It has been also proved that polyadenylation of meiotic mRNAs is essential for the selective elimination induced by Mmi1p.

本研究的目的是阐明RNA结合蛋白Mei2p和Mmi1p在裂殖酵母中调控减数分裂细胞周期的功能。结果表明，RNA聚合酶II的磷酸化对于Mei2p的表达至关重要。研究还证明，减数分裂mRNA的多聚腺苷酸化对于Mmi1p诱导的选择性消除是必需的。

项目成果

Importance of polyadenylation in the selective elimination of meiotic mRNAs in growing S. pombe cells.

• DOI: 10.1038/emboj.2010.108
• 发表时间: 2010-07-07
• 期刊: The EMBO journal
• 作者: Yamanaka S;Yamashita A;Harigaya Y;Iwata R;Yamamoto M
• 通讯作者: Yamamoto M

Contribution of dynein light intermediate and intermediate chains to subcellular localization of the dynein-dynactin motor complex in Schizosaccharomyces pombe

• DOI: 10.1111/j.1365-2443.2010.01386.x
• 发表时间: 2010-04-01
• 期刊: GENES TO CELLS
• 影响因子: 2.1
• 作者: Fujita, Ikumi;Yamashita, Akira;Yamamoto, Masayuki
• 通讯作者: Yamamoto, Masayuki

Importance of polyadenylation in the selective elimination of meiotic mRNAs in growing S.

多聚腺苷酸化在选择性消除生长中的减数分裂 mRNA 中的重要性。

• 发表时间: 2010
• 期刊: pombe cells.EMBO J. 29
• 作者: Yamanaka S.;Yamashita A.;Harigaya Y.;Iwata R.;Yamamoto M.
• 通讯作者: Yamamoto M.

In vivo analysis of cytoplasmic dynein-dynactin complex in fission yeast

裂殖酵母细胞质动力蛋白-动力蛋白复合物的体内分析

• 发表时间: 2009
• 作者: 池田信人;森川久未;中野星児;久留一郎;白吉安昭;Akira Yamashita
• 通讯作者: Akira Yamashita

Molecular basis to eliminate meiotic transcripts from mitotic cells by an RNA-binding protein Mmilp

通过RNA结合蛋白Mmilp消除有丝分裂细胞减数分裂转录物的分子基础

• 发表时间: 2009
• 作者: 青山慧;石川善則;小野山一郎;中山敬一;中山啓子;Akira Yamashita
• 通讯作者: Akira Yamashita