Functional analysis of the RNA-binding protein Mei2, which is a key player for meiosis in fission yeast

RNA 结合蛋白 Mei2 的功能分析，该蛋白是裂殖酵母减数分裂的关键参与者

• 批准号: 23570223
• 负责人: YAMASHITA Akira
• 金额: $ 3.41万
• 依托单位: Kazusa DNA Research Institute (2012-2013)The University of Tokyo (2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23570223/
• 关键词: 減数分裂; RNA結合タンパク質; 分裂酵母; non-coding RNA

The goal of this study is to understand the molecular mechanisms underlying meiosis, which is a special type of cell division for gametogenesis. Mei2 is an RNA-binding protein, which is a key player for both the induction and progression of meiosis in fission yeast. It has been shown by this study that activated Mei2 can enhance expression of a transcription factor Ste11 through a positive feedback loop involving the stress-responsive MAP kinase pathway. Furthermore, it has turned out that Mei2 is phosphorylated in mitotically growing cells depending on one of the two TOR kinase complexes in fission yeast, namely TORC1, and is degraded through the ubiquitin-proteasome pathway.

本研究的目的是了解减数分裂的分子机制，这是一种特殊类型的细胞分裂配子发生。Mei 2是一种RNA结合蛋白，在裂殖酵母减数分裂的诱导和进行中起关键作用。这项研究已经表明，激活的Mei 2可以通过涉及应激响应MAP激酶途径的正反馈回路增强转录因子Ste 11的表达。此外，已经证明Mei 2在有丝分裂生长的细胞中磷酸化，这取决于裂殖酵母中的两种TOR激酶复合物之一，即TORC 1，并通过泛素-蛋白酶体途径降解。

项目成果

Novel regulatory network for the expression of Mei2, the master regulator of meiosis

减数分裂主调控因子 Mei2 表达的新型调控网络

• 发表时间: 2011
• 作者: Akira Yamashita;Yuko Sukegawa;Yoko Otsubo;Masayuki Yamamoto
• 通讯作者: Masayuki Yamamoto

Psk1, an AGC kinase family member in fission yeast, is directly phosphorylated and controlled by TORC1 as S6 kinase

Psk1 是裂殖酵母中的 AGC 激酶家族成员，作为 S6 激酶直接被 TORC1 磷酸化并控制

• 发表时间: 2012
• 期刊: Journal of Cell Science
• 影响因子: 4
• 作者: Nakashima;A.;Otsubo;Y.;Yamashita;A.;Sato;T.;Yamamoto;M.;and Tamanoi;F.
• 通讯作者: F.

Pob1 Ensures Cylindrical Cell Shape by Coupling Two Distinct Rho Signaling Events During Secretory Vesicle Targeting

• DOI: 10.1111/j.1600-0854.2011.01190.x
• 发表时间: 2011-06
• 期刊: Traffic
• 影响因子: 4.5
• 作者: K. Nakano;M. Toya;A. Yoneda;Y. Asami;A. Yamashita;N. Kamasawa;M. Osumi;Masayuki Yamamoto

Hexanucleotide motifs meidate recruitment of the RNA elimination machinery to silent meiotic genes

六核苷酸基序介导RNA消除机制向沉默减数分裂基因的募集

• 发表时间: 2012
• 期刊: Open Biology
• 影响因子: 5.8
• 作者: Yamashita;A.;Shichino;Y.;Tanaka;H.;Hiriart;E.;Touat-Todeschini;L.;Vavasseur;A.;Ding;D-Q.;Hiraoka;Y.;Verdel;A.;and Yamamoto;M.
• 通讯作者: M.

Mmi1 RNA surveillance machinery directs RNAi complex RITS to specific meiotic genes in fission yeast

• DOI: 10.1038/emboj.2012.105
• 发表时间: 2012-05-16
• 期刊: EMBO JOURNAL
• 影响因子: 11.4
• 作者: Hiriart, Edwige;Vavasseur, Aurelia;Verdel, Andre
• 通讯作者: Verdel, Andre