Regulation of unequal cell division in animal oocyte meiosis

动物卵母细胞减数分裂中细胞分裂不均的调节

• 批准号: 20570180
• 负责人: TACHIBANA Kazunori
• 金额: $ 3.08万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20570180/
• 关键词: 不等分裂; 減数分裂; 卵母細胞; 紡錘体; 蛋白質キナーゼ; Mos; 極体; タンパク質キナーゼ; MAPK; 細胞質分裂

During meiotic maturation of animal oocytes, two successive divisions occur without an intermediate phase of DNA replication, so that haploid gametes are produced. Moreover, these two divisions are extremely asymmetric, to ensure that most of the maternal stores are retained within the oocyte. This leads to the formation of daughter cells with different sizes : the large oocyte and the small polar bodies. It is not known what signal couples the spindle pole positioning to polar body formation. We have found that Mos-MAPK-Rsk kinase pathway is required for the normal polarbody extrusion. So, we approached the question by analyzing the proteins that are phosphorylated specifically by the kinase pathway during the polarbody extrusion. We have identified some candidates for the regulators for the spindle pole positioning. Now, we are checking each candidate play a role in the asymmetric cell division.

在动物卵母细胞减数分裂成熟过程中，发生两次连续分裂，没有DNA复制的中间阶段，从而产生单倍体配子。此外，这两个分裂极其不对称，以确保大部分母体储备保留在卵母细胞内。这导致形成不同大小的子细胞：大的卵母细胞和小的极体。目前尚不清楚什么信号将主轴极定位与极体形成耦合起来。我们发现Mos-MAPK-Rsk激酶途径是正常极体挤出所必需的。因此，我们通过分析极体挤出过程中激酶途径特异性磷酸化的蛋白质来解决这个问题。我们已经确定了一些用于主轴杆定位调节器的候选者。现在，我们正在检查每个候选者在不对称细胞分裂中发挥的作用。

项目成果

イトマキヒトデの紡錘体局在の制御機構

海星纺锤体定位的控制机制

• 发表时间: 2010
• 作者: 立花和則;森雅志;熊野真弥;岸本健雄
• 通讯作者: 岸本健雄

Cyclin B-Cdkl controls pronuclear union in interphase

细胞周期蛋白 B-Cdkl 控制间期原核结合

• 发表时间: 2008
• 期刊: Curr.Biol. 18
• 作者: Tachibana;K.;et al.
• 通讯作者: et al.

Involvement of Mos-MEK-MAPK pathway in cytostatic factor (CSF) arrest in eggs of the parthenogenetic insect, Athalia rosae

• DOI: 10.1016/j.mod.2008.08.004
• 发表时间: 2008-11-01
• 期刊: MECHANISMS OF DEVELOPMENT
• 影响因子: 2.6
• 作者: Yamamoto, Daisuke S.;Tachibana, Kazunori;Hatakeyama, Masatsugu
• 通讯作者: Hatakeyama, Masatsugu

イトマキヒトデの卵成熟から受精におけるMPK2の動態

海星卵子成熟到受精过程中 MPK2 的动态

• 发表时间: 2008
• 作者: 立花和則;岸本健雄;今泉美佳;立花和則
• 通讯作者: 立花和則

Initiation of DNA replication after fertilization is regulated by p90Rsk at pre-RC/pre-IC transition in starfish eggs

海星卵中受精后 DNA 复制的启动由 p90Rsk 在 pre-RC/pre-IC 转变时调控

• 发表时间: 2010
• 期刊: Proc. Natl. Acad. Sci. USA
• 作者: Tachibana;K.;Mori;M.;Matsuhira;T.;Karino;T.;Inagaki;T.;Nagayama;A.;Nishiyama;A.;Hara;M.;and Kishimoto;T
• 通讯作者: T