Novel DNA replication checkpoint in the assembly of DNA replication complex

DNA复制复合物组装中的新型DNA复制检查点

• 批准号: 15570155
• 负责人: TACHIBANA Kazunori
• 金额: $ 2.43万
• 依托单位: TOKYO INSTITUTE OF TECHNOLOGY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15570155/
• 关键词: DNA REPLICATION; replication licensing; Mcm; Cdc45; Checkpoint; Cell cycle; starfish; fertilization; MCM; Cdc45; Cdc7; Cdk2; Cyclin E

The regulation of the initiation of DNA replication in eukaryotic cells has been studied extensively the last enigmatic question is how cells form pre-initiation complex onto the DNA replication origins. The major factors involved in the assembly of the pre-initiation complex are Sld2-Dpb11,Sld3,GINS (Go, Ichi, Ni, and San), and S-phase kinases (Cdk;cyclin-dependent kinase, and Ddk,;Dbf4-dependent kinase). Recently, Wolter et al. reported that protein phosphatase 2A (PP2A) controls the pre-initiation complex in Xenopus cell-free system (J.Biol.Chem.2002). We have tested whether PP2A controlled the pre-initiation complex in starfish eggs. Neither the catalytic subunit of PP2A ligated with nuclear localization signal peptide sequence was ectopically expressed in starfish eggs nor okadaic acid, potent specific PP2A inhibitor was applied to starfish fertilized eggs, had significant effect to the initiation of the DNA replication.

真核细胞中 DNA 复制起始的调控已被广泛研究，最后一个神秘的问题是细胞如何在 DNA 复制起点上形成预起始复合物。参与预起始复合物组装的主要因素是 Sld2-Dpb11、Sld3、GINS（Go、Ichi、Ni 和 San）和 S 期激酶（Cdk；细胞周期蛋白依赖性激酶和 Ddk；Dbf4 依赖性激酶）。最近，沃尔特等人。据报道，蛋白磷酸酶 2A (PP2A) 控制非洲爪蟾无细胞系统中的预起始复合物 (J.Biol.Chem.2002)。我们测试了 PP2A 是否控制海星卵中的预起始复合物。与核定位信号肽序列连接的PP2A催化亚基在海星卵中均未异位表达，强效特异性PP2A抑制剂冈田酸应用于海星受精卵时，对DNA复制的启动具有显着影响。

项目成果

Distinct regulators for Plk1 activation in starfish meiotic and early embryonic cycles

• DOI: 10.1093/emboj/cdg535
• 发表时间: 2003-10-15
• 期刊: EMBO JOURNAL
• 影响因子: 11.4
• 作者: Okano-Uchida, T;Okumura, E;Kishimoto, T
• 通讯作者: Kishimoto, T

Okano, Uchida et al.: "Distinct regulators for PID1 activation in starfish meiotic and early embryonic cycles"The EMBO Journal. 22(20). 5633-5642 (2003)

Okano, Uchida 等人：“海星减数分裂和早期胚胎周期中 PID1 激活的不同调节因子”EMBO 杂志。

発生における細胞増殖制御

发育过程中细胞增殖的控制

• 发表时间: 2004
• 作者: 山口政光;加藤容子
• 通讯作者: 加藤容子

竹内隆, 岸本健雅編 立花和則(分担): "発生における細胞増殖制御"シュプリンガーフェアラーク東京. 198 (2004)

Takashi Takeuchi、Takemasa Kishimoto（编辑）Kazunori Tachibana（撰稿人）：“发育中的细胞增殖控制”Springer Verlag Tokyo 198 (2004)。

PDK1 is required for the hormonal signaling pathway leading to meiotic resumption in starfish oocytes

• DOI: 10.1016/j.ydbio.2004.08.036
• 发表时间: 2004-12-15
• 期刊: DEVELOPMENTAL BIOLOGY
• 影响因子: 2.7
• 作者: Hiraoka, D;Hori-Oshima, S;Kishimoto, T
• 通讯作者: Kishimoto, T