Screening and functional analysis for virulence factor of phytoplasma

植原体毒力因子的筛选及功能分析

• 批准号: 20580044
• 负责人: OSHIMA Kenro
• 金额: $ 3万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20580044/
• 关键词: ファイトプラズマ; ゲノム; 病原性因子; 病原性因

Phytoplasmas are bacterial plant pathogens and bring about dramatic changes in plant development, including witches' broom, dwarfism, proliferation, and phyllody. In this study, we focused on the secreted proteins encoded in the phytoplasma genome, and screened the virulence factor of phytoplasma. As a result, we found that a single virulence factor, named TENGU, induced witches' broom and dwarfism. The functional analysis of TENGU revealed that TENGU inhibits auxin-related pathways, thereby affecting plant development.

植原体是一种细菌性植物病原体，它能引起植物生长发育的显著变化，包括丛枝病、矮化、增殖和叶状体。本研究以植原体基因组中编码的分泌蛋白为研究对象，筛选植原体毒力因子。结果发现，一个名为TENGU的毒力因子能诱导丛枝病和矮化病。TENGU的功能分析表明，TENGU抑制生长素相关的途径，从而影响植物的发育。

项目成果

ファイトプラズマの昆虫伝搬能喪失株におけるプラスミドの退行的進化

昆虫传播植原体菌株中质粒的回归进化

• 发表时间: 2010
• 作者: Kume;T.;Onozawa;Y.;Komatsu;H.;Tsuruta;K.;Shinohara;Y.;Umebayashi;T.;Otsuki;K.;石井佳子・菅原杏子・小島信子・石井良裕・煉谷裕太朗・柿澤茂行・星朱香・姫野未紗子・大島研郎・難波成任;石井佳子・煉谷裕太郎・柿澤茂行・大島研郎・難波成任
• 通讯作者: 石井佳子・煉谷裕太郎・柿澤茂行・大島研郎・難波成任

Sequence diversity and positive selection of a surface membrane protein of phytoplasma.

植原体表面膜蛋白的序列多样性和正选择。

• 发表时间: 2008
• 作者: Kakizawa S.;Ishii Y.;Hoshi A.;Jung H.Y.;Oshima K.;Namba S.
• 通讯作者: Namba S.

Positive selection on a surface membrane protein and insect vector specificity of phytoplasma.

植原体表面膜蛋白和昆虫载体特异性的正选择。

• 发表时间: 2008
• 作者: Kakizawa S;Ishii Y;Hoshi A;Jung HY;Oshima K;Namba S
• 通讯作者: Namba S

Cloning and characterization of the antigenic membrane protein (Amp) gene and in situ detection of Amp from malformed flowers infected with Japanese hydrangea phyllody phytoplasma

• DOI: 10.1094/phyto-98-7-0769
• 发表时间: 2008-07-01
• 期刊: PHYTOPATHOLOGY
• 影响因子: 3.2
• 作者: Arashida, R.;Kakizawa, S.;Namba, S.
• 通讯作者: Namba, S.

Cloning and Molecular Evolutionary Analysis of Genes Encoding Immunodominant Membrane Proteins of Phytoplasma.

植原体免疫显性膜蛋白编码基因的克隆和分子进化分析。

• 发表时间: 2010
• 作者: Kakizawa;S ; Neriya;Y ; lshii;Y ; Hoshi;A ; Kojima;N ; Sugawara;K ; Okano.Y ; Maeiima.K
• 通讯作者: K ; Okano.Y ; Maeiima.K