Studies on the types of the taste cell and taste perception.

味觉细胞类型和味觉感知的研究。

• 批准号: 20580128
• 负责人: HAYASHI Yukako
• 金额: $ 3.08万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20580128/
• 关键词: 味覚; マウス; 情報変換; 苦味; 味細胞; 情報伝達; カフェイン; 免疫染色; カルシウム動員; カルシウムチャネル; カルシウムリリース; ガストジューシン

There are a lot of unsolved issues about the pathway from taste cells to taste cells or taste nerves. Therefore, we analyzed the caffeine transduction mechanism of mouse using behavioral test, calcium imaging, and immunocytochemistry. The responsiveness of taste cells to caffeine, ATP, and high concentration of K^+ were examined by calcium imaging followed by immunocytochemistry to detect expression of G_α-gustducin and IP_3R3 which are known as type II cell markers. As a result, caffeine stimulated taste cells both type II and the other type of cells. A part of type II cells was depolarized by high concentration of K^+, and taste cells which responded to caffeine and high K^+ existed. Therefore we concluded there are voltage-gated calcium channels in subset of type II cells. Also, ATP stimulates both type II and other type of cells. Considering the report that ATP is released from type II cells, it seems to play an autocrine role in taste cells.

关于味觉细胞到味觉细胞或味觉神经的通路，还有许多尚未解决的问题。因此，我们采用行为学、钙显像和免疫细胞化学等方法分析了小鼠咖啡因的转导机制。通过钙显像和免疫细胞化学检测味觉细胞对咖啡因、ATP和高浓度K^+的反应性，检测II型细胞标志物G_α-gustducin和IP_3R3的表达。结果，咖啡因刺激了味觉细胞II型和其他类型的细胞。高浓度K^+使部分II型细胞去极化，存在对咖啡因和高K^+有反应的味觉细胞。因此，我们得出结论，在II型细胞亚群中存在电压门控钙通道。此外，ATP刺激II型和其他类型的细胞。考虑到ATP从II型细胞释放的报道，它似乎在味觉细胞中起自分泌作用。

项目成果

Signalling mechanisms in mouse bitter responsive taste cells.

小鼠苦味反应味细胞的信号传导机制。

• 发表时间: 2009
• 期刊: Neuroreport 20(10)
• 作者: Narukawa M;Minamisawa E;Hayashi Y.
• 通讯作者: Hayashi Y.

L-Theanine Elicits an Umami Taste with Inosine 5′-Monophosphate

• DOI: 10.1271/bbb.80328
• 发表时间: 2008-11-01
• 期刊: BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
• 影响因子: 1.6
• 作者: Narukawa, Masataka;Morita, Kanako;Hayashi, Yukako
• 通讯作者: Hayashi, Yukako

味細胞におけるカフェイン味刺激伝達経路の解析

味觉细胞中咖啡因味觉刺激传递途径分析

• 发表时间: 2009
• 期刊: 日本味と匂い学会誌 16(3)
• 作者: 藤戸洋聡;谷口友朗;北田亮;呉性姫;松村康生;林由佳子
• 通讯作者: 林由佳子

Characterization of umami receptor and coupling G protein in mouse taste cells.

小鼠味觉细胞中鲜味受体和偶联 G 蛋白的表征。

• 发表时间: 2008
• 期刊: Neuroreport 19
• 作者: Narukawa;M.
• 通讯作者: M.

Evaluation of Umami substance and the characterization of umami receptor and coupling G protein

鲜味物质的评价以及鲜味受体和偶联G蛋白的表征

• 发表时间: 2010
• 作者: 北田亮;藤戸洋聡;呉性姫;林由佳子;林由佳子;林由佳子
• 通讯作者: 林由佳子