A trial for establishing methods for predicting flowering and bearing stages of bload-leaved trees by functional analysis of flowering mutants from Japanese beech tree.

通过对日本山毛榉开花突变体的功能分析，建立预测白叶树开花和结果阶段的方法的试验。

• 批准号: 20580168
• 负责人: OHMIYA Yasunori
• 金额: $ 3万
• 依托单位: Forestry and Forest Products Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20580168/
• 关键词: 遺伝子; 樹木; 発現制御; 森林工学; ブナ; 植物

We have isolated Arabidopsis orthologs, constans (CO) and flowering locus T (FT) genes from Japanese beech tree, which are mainly function in flower transition, and their full sequences were identified. Real-Time PCR analysis revealed that CO transcripts are closely related to flowering initiation with high expression level of CO mRNAs during the period in May, a year before flowering. Transgenic Arabidopsis overexpressing CO product represented early flowering phenotype, this is a result of revealing its flowering enhancement function of CO. Their comparisons of nucleotide sequences were performed among 38 clones collected from Tohoku area. It seemed likely that there are possibilities of insertion/deletion including nucleotide polymorphisms (SNPs) within several FT genes that might influence its function, while there are no differences among CO sequences. Further analyses of FT including SNPs and producing overexpressing lines are ongoing.

我们从日本山毛榉中分离了拟南芥同源基因constans（CO）和开花位点T（FT），这两个基因主要在花转变中起作用，并对它们的全序列进行了鉴定。实时荧光定量PCR分析表明，CO转录本与开花启动密切相关，在开花前一年的5月份，CO mRNA表达量较高。过量表达CO产物的转基因拟南芥表现为早花表型，这是揭示其CO促进开花功能的结果。这似乎是可能的，有插入/删除的可能性，包括核苷酸多态性（SNP）的几个FT基因，可能会影响其功能，而CO序列之间没有差异。FT的进一步分析包括SNP和产生过表达系正在进行中。

项目成果

毎年花を着ける?

你每年都戴花吗？

• 发表时间: 2009
• 期刊: ブナのお話、みどりの東北 65巻
• 作者: 郷雅和;岡浩平;宮浦徹;吉崎真司;原秀穂;大宮泰徳
• 通讯作者: 大宮泰徳

毎年花を着ける? ブナのお話

山毛榉树每年都开花吗？

• 发表时间: 2009
• 期刊: みどりの東北 65
• 作者: 吉崎真司;他;原秀穂;大澤正嗣;大宮泰徳
• 通讯作者: 大宮泰徳

ブナ花成関連遺伝子FcCO, FcFTの単離と発現解析

山毛榉开花相关基因FcCO和FcFT的分离及表达分析

• 发表时间: 2011
• 作者: STEPHAN M. BLANK;HIDEHO HARA;JOZSEF MIKULAS;GYORGY CSOKA;CONSTANTIN CIORNEI;RAOUL CONSTANTINEANU;IRINEL CONSTANTINEANU;LADISLAV ROLLER;EWALD ALTENHOFER;TOMASZ HUFLEJT and GABOR VETEK;吉崎真司;大澤正嗣;大宮泰徳
• 通讯作者: 大宮泰徳

ブナ花成研究事始め

开始山毛榉开花研究

• 发表时间: 2009
• 期刊: 林木の育種 233巻
• 作者: 吉崎真司;他;Masashi Ohsawa and Toshio Shimokawa;原秀穂;大宮泰徳
• 通讯作者: 大宮泰徳

ブナ花成関連遺伝子の単離と解析

山毛榉开花相关基因的分离与分析

• 发表时间: 2010
• 作者: Shinohara A.;Hara H.;J.-W.Kim;大澤正嗣;大宮泰徳
• 通讯作者: 大宮泰徳