Unicellular diazotrophic cyanobacteira : development of immunocytochemical methods for nitrogenase detection and analysis of their role in Kuroshio Waters.

建立了一种适用于检测海水中单细胞固氮蓝藻的免疫细胞化学方法。利用已开发的方法，在黑潮水域(爱贤县、宫城县和台湾南部海域)采集的海水中检测到单细胞重氮营养蓝藻。利用固氮酶和16SrRNA基因的DNA扩增技术对单细胞重氮营养蓝藻进行了检测。通过免疫细胞化学和DNA检测方法，在爱媛和宫城附近的水域中几乎没有检测到重氮型单细胞蓝藻。台湾海域单细胞固氮蓝藻数量丰富，细胞密度在0.5~1.5×103/L之间，从台湾海域和台湾海域成功分离到两株重氮藻。经鉴定为Gloeothess spp.(UWA1)和蓝藻(Cyanothess spp.)(Tw3)从16S rRNA和固氮酶(NifH)基因的部分序列及其形态。

Immunocytochemical methods that is applicable for detection of unicellular diazotrophic cyanobacteria in sea waters have been developed. Using developed methods, unicellular diazotrophic cyanobacteria were detected in sea waters collected from Kuroshio waters (off Ehime Prefecture, off Miyagi prefecture and off from South Taiwan). Detection of unicellular diazotrophic cyanobacteria was also tried using DNA-amplification of nitrogenase and 16SrRNA genes. Few diazotrophic unicellular cyanobacteria were detected by both immunocytochemical and DNA-based methods in waters from off Ehime and Miyagi. Unicellular diazotrophic cyanobacteria was abundant in waters from off Taiwan : cell densities were 0.5 to 1.5×103/L. Two diazotrophic cyanobacteria were successfully isolated from off Ehime (UWA1) and Taiwan (TW3). They were identified as Gloeothece spp. (UWA1) and Cyanothece spp. (TW3) from the partial sequences of 16S rRNA and nitrogenase (nifH) genes and their morphology.