N_2-fixation in marine cyanophyte Trichodesmium cultured under artificial conditions

人工条件下海洋蓝藻Trichodesmium的N_2固定

基本信息

项目摘要

Marine, pelagic cyanophyte, Trichodesmium, able to fix N_2 under aerobic conditions in the light. Mechanisms for the maintenance of N_2-fixation activity in Trichodesmium under aerobic conditions were studied. Results and further view are as follow : 1. N_2-fixation of Trichodesmium is regulated at least two different levels, biosynthesis of nitrogenase and post-translational modification of the Fe-protein of nitrogenase. 2. Examination by immuno-electron microscopy showed that Trichodesmium did not develop the special cells (heterocysts) which were the site of aerobic N_2-fixation in cyanophyte. Almost all cells contain nitrogenase. 3. The high homology of the primary structure of the Fe-protein of nitrogenase between Trichodesmium and other N_2-fixing organisms was found by sequencing of the structure gene (nif H). Sequencing of the structure genes of the FeMo-protein of nitrogenase (nif D,H) is under progress. 4. N_2-fixation activity of Trichodesmium is maintained by the light. Activity was abruptly lost when cells were transferred from light to the dark. The Fe-protein of nitrogenase became inactive form in the dark, and light was required to change from inactive to active form. Protein synthesis inhibitors inhibit the activation process by light. These results suggest that (1)light is required to maintain the nitrogenase activity not only to supply ATP and/or reductant by photosynthesis but also keeping the Fe-protein active form, and that (2)proteinous factor(s) which turns over rapidly acts during the activation process. Determination of proteinous factors(s) and further analysis of the effect of light on transcriptional and translational levels of nitrogenase are required.
海洋,远洋蓝藻,trichodesium,能够在光照下的好氧条件下固定N_2。研究了在好氧条件下木霉维持固氮活性的机理。结果和进一步的看法如下:木霉的n_2固定至少受到两个不同水平的调控,即氮酶的生物合成和氮酶铁蛋白的翻译后修饰。2. 免疫电镜检查显示,赤霉病菌没有形成蓝藻中作为需氧氮固定位点的特殊细胞(异囊)。几乎所有的细胞都含有氮酶。3. 通过对结构基因(nif H)的测序,发现木霉菌与其他固氮生物的氮酶铁蛋白一级结构高度同源。氮酶的femo蛋白(nif D,H)结构基因的测序正在进行中。4. 光维持木霉的n_2固定活性。当细胞从光照环境转移到黑暗环境时,活动突然消失。氮酶铁蛋白在黑暗条件下变为失活状态,光照条件下由失活状态变为活性状态。蛋白质合成抑制剂抑制光的激活过程。这些结果表明:(1)维持氮酶活性不仅需要光来通过光合作用提供ATP和/或还原剂,还需要光来保持铁蛋白的活性形式;(2)在激活过程中快速翻转的蛋白质因子(s)起作用。需要测定蛋白质因子和进一步分析光对氮酶转录和翻译水平的影响。

项目成果

期刊论文数量(9)
专著数量(0)
科研奖励数量(0)
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专利数量(0)
Jonathan P.Zehr: "Arrangement of nitrogenase structural genes in an aerobic filamentous nonheterocystous Cyanobacterium." J.Bact.137. 7055-7058 (1991)
Jonathan P.Zehr:“需氧丝状非异囊蓝细菌中固氮酶结构基因的排列。”
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J.P..Zehr: "Arrengement of nitrogenase structural genes in an aerobic filamentous non-heterocystous cyanobacterium. (1991)" J.Bacteriol. 173. 7055-58 (1991)
J.P..Zehr:“需氧丝状非异囊蓝细菌中固氮酶结构基因的排列。(1991)”J.Bacteriol。
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Ohki,K.,Rueter,J.G.,Falkowski,P.G.,Fujita,Y.(Ed.Mauchline,J.,Nemoto,T.): "Experimental study of Trichodesmium,a nitrogenーfixing cyanophyte in tropical and subtropical sea areas." Marine Biology,Its accomplishment and feature prospect. 209-219 (1991)
Ohki, K.、Rueter, J.G.、Falkowski, P.G.、Fujita, Y.(Ed. Mauchline, J.、Nemoto, T.):“热带和亚热带海域固氮蓝藻Trichodesmium 的实验研究。”海洋生物学,其成就与特色展望。209-219(1991)
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J.P.Zehr: "Unique modification of adenine in genomic DNA of the marine cyanobacterium Trichodesmium sp. strain NIBB1067" J.Bacteriol. 173. 7059-62 (1991)
J.P.Zehr:“海洋蓝藻 Trichodesmium sp. 菌株 NIBB1067 基因组 DNA 中腺嘌呤的独特修饰”J.Bacteriol。
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Ohki,K.,Zehr,P.J.,Falkouki,P.G.,Fujita,Y.: "Regulation of nitrogenーfixation by different nitrogen sources in marine nonーheterocystous cyanobacterium Trichodesmium sp." Arch.Microbiol.
Ohki, K.、Zehr, P.J.、Falkouki, P.G.、Fujita, Y.:“海洋非异囊蓝藻 Trichodesmium sp. 中不同氮源的固氮调节。”
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OHKI Kaori其他文献

OHKI Kaori的其他文献

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{{ truncateString('OHKI Kaori', 18)}}的其他基金

Molelcular mechanisms of exopolysaccharide production in edible cyanobacterium, Aphanothece sacrum (Suizenji-Nori)
食用蓝藻 Aphanothece sacrum (Suizenji-Nori) 产生胞外多糖的分子机制
  • 批准号:
    15K07557
  • 财政年份:
    2015
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Unicellular diazotrophic cyanobacteira : development of immunocytochemical methods for nitrogenase detection and analysis of their role in Kuroshio Waters.
单细胞固氮蓝藻:开发用于固氮酶检测的免疫细胞化学方法并分析其在黑潮水域中的作用。
  • 批准号:
    20580208
  • 财政年份:
    2008
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Lysogenic cyanophages; molecular mechanisms of lysogeny and their role in marine ecosystems
溶原性噬藻体;
  • 批准号:
    09490034
  • 财政年份:
    1997
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)

相似海外基金

The Trichodesmium consortium: the marine N-cycle at the microscale?
Trichodesmium 财团:微观尺度的海洋氮循环?
  • 批准号:
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Collaborative Research: Dissolved Phosphorus Processing by Trichodesmium Consortia: Quantitative Partitioning, Role of Microbial Coordination, and Impact on Nitrogen Fixation
合作研究:Trichodesmium Consortia 的溶解磷处理:定量分配、微生物协调的作用以及对固氮的影响
  • 批准号:
    1332898
  • 财政年份:
    2013
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    $ 1.15万
  • 项目类别:
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Collaborative Research: Dissolved Phosphorus Processing by Trichodesmium Consortia: Quantitative Partitioning, Role of Microbial Coordination, and Impact on Nitrogen Fixation
合作研究:Trichodesmium Consortia 的溶解磷处理:定量分配、微生物协调的作用以及对固氮的影响
  • 批准号:
    1332912
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EAGER: Gene expression patterns in high C02-adapted Trichodesmium
EAGER:高CO2适应的Trichodesmium中的基因表达模式
  • 批准号:
    1143760
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Quantification of Trichodesmium spp. vertical and horizontal abundance patterns and nitrogen fixation in the western North Atlantic
Trichodesmium spp 的定量。
  • 批准号:
    0925284
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Introns and inteins in Trichodesmium
Trichodesmium 中的内含子和内含子
  • 批准号:
    41823-2005
  • 财政年份:
    2009
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Discovery Grants Program - Individual
Multiscale Structure-Function Relationships of Collagen in the Marine Cyanobacterium Trichodesmium erythraeum
海洋蓝藻红毛藻中胶原蛋白的多尺度结构-功能关系
  • 批准号:
    0900101
  • 财政年份:
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Introns and inteins in Trichodesmium
Trichodesmium 中的内含子和内含子
  • 批准号:
    41823-2005
  • 财政年份:
    2008
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Discovery Grants Program - Individual
Fe Acquisition by Trichodesmium: Role of the Associated Microbial Community
毛藻对铁的获取:相关微生物群落的作用
  • 批准号:
    0838213
  • 财政年份:
    2007
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Standard Grant
Introns and inteins in Trichodesmium
Trichodesmium 中的内含子和内含子
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    41823-2005
  • 财政年份:
    2007
  • 资助金额:
    $ 1.15万
  • 项目类别:
    Discovery Grants Program - Individual
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