Mechanisms of sexual dimorphism of IFNτ secretion in bovine blastocyst

牛囊胚分泌IFNτ的性别二态性机制

• 批准号: 20580333
• 负责人: KIMURA Koji
• 金额: $ 3万
• 依托单位: National Agricultural Research Organization
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20580333/
• 关键词: ウシ; 胚; インターフェロン; 性; 転写因子; インターフェロン・タウ; 性差; インターフェロンタウ; 遺伝子転写因子

Sexual dimorphic expression of IFNτ of bovine embryo was not caused via the sexual difference of expression of transcription factors, which affect IFNτ gene expression. However, the expression of Dnmt3a, one of DNA methyltransferases, of bovine male blastocysts was significantly higher than that of female embryos. Moreover, addition of 5-azadeoxycytidene, an inhibitor of DNA methyltransferase, to the culture medium of bovine embryo abolished the sexual dimorphic secretion of IFNτ from bovine embryos. These results suggest that the DNA methylation of bovine embryo genome might be involved in the occurrence of sexual dimorphic expression of IFNτ in bovine embryos.

影响IFNτ基因表达的转录因子的性别差异并不会导致牛胚胎中IFNτ基因的性别二态表达。而DNA甲基转移酶之一Dnmt3a在牛雄性囊胚中的表达显著高于雌性囊胚。此外，在牛胚胎培养基中添加DNA甲基转移酶抑制剂5-azadeoxycytidene，可以抑制牛胚胎中IFNτ的两性二态分泌。这些结果提示，牛胚胎基因组DNA甲基化可能参与了牛胚胎中IFNτ有性二态表达的发生。

项目成果

Expression of bovine interferon-tau variants according to sex and age of conceptuses.

根据性别和受孕年龄的牛干扰素-tau 变体的表达。

• DOI: 10.1016/j.theriogenology.2009.01.017
• 发表时间: 2009
• 期刊: Theriogenology
• 影响因子: 2.8
• 作者: Walker,AngelaM;Kimura,Koji;Roberts,RMichael
• 通讯作者: Roberts,RMichael

Effects of modification of in vitro fertilization techniques on the sex ratio of the resultant bovine embryos.

• DOI: 10.1016/j.anireprosci.2007.03.006
• 发表时间: 2008-05
• 期刊: Animal reproduction science
• 影响因子: 2.2
• 作者: H. Iwata;H. Shiono;Y. Kon;K. Matsubara;K. Kimura;T. Kuwayama;Y. Monji

Effect of transfer of cattle elongating embryo to a repeat breeder cow on pregnancy rate and incidence of a return of estrus

将牛延长胚胎移植至重复种牛对妊娠率和回情发生率的影响

• 发表时间: 2011
• 作者: Iwata H;Shiono H;Kon Y;Matsubara K;Kimura K;Kuwayama T;Monji Y.;Kimura K
• 通讯作者: Kimura K

Nutritional skewing of conceptus sex in sheep: effects of a maternal diet enriched in rumen-protected polyunsaturated fatty acids (PUFA)

• DOI: 10.1186/1477-7827-6-21
• 发表时间: 2008-06-09
• 期刊: REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
• 影响因子: 4.4
• 作者: Green, Mark P.;Spate, Lee D.;Roberts, R. Michael
• 通讯作者: Roberts, R. Michael

ウシ胚盤胞期胚におけるIFN-τ発現に関わる転写因子の遺伝子発現について

牛囊胚期胚胎中参与IFN-τ表达的转录因子的基因表达

• 发表时间: 2010
• 作者: Iwata H;Shiono H;Kon Y;Matsubara K;Kimura K;Kuwayama T;Monji Y.;Kimura K;木村康二・松山秀一
• 通讯作者: 木村康二・松山秀一