Effects of metabolism of purine derivatives on bovine embryo development

研究了嘌呤衍生物代谢对牛胚胎发育的影响。当从头嘌呤合成被抑制时，母体到合子转换（MZT）后的胚胎发育严重受损。此外，当嘌呤衍生物（腺嘌呤或次黄嘌呤）在嘌呤从头合成抑制剂存在下补充时，抑制作用消失。这些结果表明，嘌呤是必需的牛胚胎发育后MZT。然而，过量的嘌呤衍生物（尤其是腺嘌呤）会使牛胚胎发育恶化。受精后，过量的腺嘌呤使胚胎卵裂停止，核碎裂。

The effects of metabolism of purine derivatives on bovine embryo development were investigated. When de novo purine synthesis was inhibited, the embryo development after maternal-to-zygotic transition (MZT) was severely compromised. Moreover, when the purine derivatives (adenine or hypoxanthine) were supplemented in the presence of the inhibitor for de novo purine synthesis, the inhibitory effect disappeared. These results suggested that purines are necessary for bovine embryo development after MZT. However, excess amount of purine derivatives (especially adenine) deteriorated bovine embryo development. In the presence of excess amount of adenine after fertilization, the cleavage of the embryo was arrested and the fragmentation of nuclear was induced.