Effects of metabolism of purine derivatives on bovine embryo development

研究了嘌呤类化合物代谢对牛胚胎发育的影响。当从头合成被抑制时，母体向受精卵转变(MZT)后的胚胎发育受到严重影响。此外，当嘌呤类化合物(腺嘌呤或次黄嘌呤)与从头合成嘌呤的抑制剂同时存在时，抑制作用消失。这些结果表明，嘌呤在牛胚胎发育过程中是必需的。然而，过量的嘌呤衍生物(特别是腺嘌呤)会恶化牛胚胎的发育。在受精后过量的腺嘌呤存在下，胚胎的卵裂被阻止，并导致核的碎裂。

The effects of metabolism of purine derivatives on bovine embryo development were investigated. When de novo purine synthesis was inhibited, the embryo development after maternal-to-zygotic transition (MZT) was severely compromised. Moreover, when the purine derivatives (adenine or hypoxanthine) were supplemented in the presence of the inhibitor for de novo purine synthesis, the inhibitory effect disappeared. These results suggested that purines are necessary for bovine embryo development after MZT. However, excess amount of purine derivatives (especially adenine) deteriorated bovine embryo development. In the presence of excess amount of adenine after fertilization, the cleavage of the embryo was arrested and the fragmentation of nuclear was induced.