Analysis of a novel protein complex involved in RNAi and maintenance of chromosome integrity in higher eukaryote

本研究旨在阐明一种新的线虫RNAi因子DRH-3的分子功能，它最初是在我之前的工作中发现的。在这项研究中，我通过酵母双杂交分析成功地鉴定了一个与DRH-3相互作用的新的E1蛋白，表明DRH-3可能通过与线虫中的E1蛋白相互作用而在RNAi途径中发挥作用。因此，E1蛋白可能是阐明DRH-3蛋白复合体分子机制的重要线索。此外，表达谱分析表明，DRH-3定位于特定的细胞结构域，并在生殖系形成中发挥作用。

This study has aimed to clarify the molecular function of a novel nematode RNAi factor DRH-3 that was originally found in my previous work. In this study, I successfully identified a novel E1 protein that interacts with DRH-3 by comprehensive yeast two-hybrid analyses, suggesting that DRH-3 likely functions in the RNAi pathway by interacting with E1 protein in C. elegans. Thus, E1 protein could be an important clue to clarify the molecular mechanism of the DRH-3-containing protein complex. In addition, the expression profile analyses suggested that DRH-3 localizes in particular cellular domains and plays a role in germ-line formation.