权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Analysis of functional significance of lipids in receptors for vision, hearing and olfaction by way of functional analysis of fatty acid binding proteins

通过脂肪酸结合蛋白的功能分析来分析视觉、听觉和嗅觉受体中脂质的功能意义

基本信息

批准号：
20590180
负责人：
SAINO Sachiko
金额：
$ 3万
依托单位：
Tohoku Bunka Gakuen University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2008
资助国家：
日本
起止时间：
2008 至 2010
项目状态：
已结题

项目摘要

In the retina of normal adult mice, B-FABP was mainly localized in the cone photoreceptor cells, H-FABP in some populations of amacrine/bipolar/horizontal interneurons, and E-FABP in ganglion cells, suggesting the discrete functional involvement of FABPs and their ligands, FAs in the photoreception and photo-transmission. In addition, A-FABP-like immunoreactivity was located in resident microglia of normal retinae. In damaged retinae following photic injury, E-FABP was intensely localized in invasive macrophages, allowing discrete identification of the resident microglia and invasive macrophages by A- and E-FABP immunoreactivity, respectively. In the inner ear of normal adult mice, H (heart- type) -FABP was localized in inner and outer pillar cells and outer phalangeal cells, while B (brain-type)-FABP was localized in border cells and cells of Hensen, and fibrocytes in the spiral limbus and spiral prominence. However, no hair cells expressed any species of FABPs. Althoug the FABP species and/or their ligands, FAs, is suggested to play important roles in the regulation of the hearing function, mutant mice with deletion of the gene for either H- or B-FABP did not show any impairment of the hearing ability. In addition to the two sensory organs, E-FABP was expressed/localized in most, if not all, populations of the dendritic cells in the subepithelial domes, follicles and interfollicular regions of Peyer's patches and presumptive macrophages in their germinal centers, and all M cells in the follicle-associated epithelium of mouse intestine. The immunoreactivity in both of the cell populations makes it easy to recognize the accumulation of DCs in the subepithelial domes in close proximity to the base of M cells, which is essential for luminal antigens to be transported to Peyer's patches. E-FABP may play some important roles in the mucosal immune reaction through Peyer's patches and associated structures.

在正常成年小鼠视网膜中，B-FABP主要定位于视锥感光细胞，H-FABP定位于部分无长突/双极/水平间神经元，E-FABP定位于神经节细胞，提示FABP及其配体、FAs参与光接收和光传递。此外，A-FABP样免疫反应位于正常视网膜的驻留小胶质细胞中。在光损伤后受损的视网膜中，E-FABP强烈定位于侵袭性巨噬细胞，通过A-和E-FABP免疫反应分别识别驻留的小胶质细胞和侵袭性巨噬细胞。在正常成年小鼠内耳，H(心型)-FABP定位于内外柱状细胞和外指节细胞，B(脑型)-FABP定位于边缘细胞和Hensen细胞，纤维细胞定位于螺旋缘和螺旋突起。然而，没有毛细胞表达任何种类的FABP。尽管FABP的种类和/或其配体FAs被认为在听力调节中起重要作用，但H-FABP或B-FABP基因缺失的突变小鼠并未表现出任何听力损害。除这两个感觉器官外，E-FABP在小鼠肠道上皮下穹隆、滤泡和滤泡间区的树突状细胞群以及生发中心的巨噬细胞群以及小鼠肠道滤泡相关上皮中的所有M细胞都有表达/定位。这两种细胞群中的免疫反应使人们很容易识别在靠近M细胞底部的上皮下穹隆中聚集的DC，这是腔抗原运输到Peyer‘s斑块所必需的。E-FABP可能通过Peyer‘s斑块及相关结构在粘膜免疫反应中发挥重要作用。