Dynamical properties of mouse ES-cell derived cardiomyocytes during differentiation: insights from bifurcation analysis based on nonlinear dynamical system theory

点击翻译按钮获取中文摘要

The aim of this study was to elucidate the dynamical mechanisms of generation and regulation of pacemaker activity during differentiation of mouse ES-cell derived cardiomyocytes. We have constructed nonlinear dynamical systems (nonlinear ordinary differential equations) of the ES-cell derived cardiomyocytes (sinoatrial node-type, atrial-type, and ventricular-type) on the basis of electrophysiological data. Analyzing the bifurcation structures (ways of generating or abolishing pacemaker activity) of these model systems, we have clarified that the emergence and abolition of pacemaker activity during differentiation of the cardiomyocytes are bifurcation phenomena, and that both sarcolemmal ionic channel currents and intracellular Ca^<2+> dynamics are involved in occurrence of the bifurcations.