Search of new therapeutic agents for drug dependence and analysis of the inherited difference in patients with drug dependence.

药物依赖新治疗药物的寻找及药物依赖患者遗传差异分析。

• 批准号: 20590269
• 负责人: KATSURA Masashi
• 金额: $ 3万
• 依托单位: Kawasaki Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590269/
• 关键词: 薬物依存; アルコール; L型高電位開口性カルシウムチャネル; 熱ショック蛋白; 神経細胞; 鉄代謝; physical dependence; psychological dependence; L-type high voltage-gated Ca^<2+>channel; stored operated Ca^<2+>channel; morphine; alcohol; Fe^<2+>content; L-type high voltage-gated Ca^<2+>

We investigated the detailed and new common mechanisms underling the accentuation of L-type high voltage-gated calcium channels function in establishment of psychological and physical dependence. Phosphorylation of heat shock factor and following increase in heat shock proteins 70 (HSP70) were observed in sustained ethanol-treated neuronal cells. In addition, intracellular ferrous iron concentrations were also significant increased after long-term exposure of ethanol. These results suggest the possibility that increase in intracellular HSP70 expressions and ferrous iron concentrations are new target for mechanism of drug dependence.

我们研究了L类高电压门控钙通道在建立心理和身体依赖中作用的详细和新的共同机制。在持续乙醇处理的神经细胞中，观察到热休克因子的磷酸化和热休克蛋白70(HSP70)的增加。此外，长期接触乙醇后，细胞内亚铁浓度也显著升高。这些结果提示，细胞内HSP70表达增加和亚铁浓度升高可能是药物依赖机制的新靶点。

项目成果

Direct evidence for the up-regulation of Vps34 regulated by PKCg during short-term treatment with morphine.

吗啡短期治疗期间 PKCg 调节的 Vps34 上调的直接证据。

• 发表时间: 2009
• 期刊: Synapse 63
• 作者: Shibasaki M;Kurokawa K;Katsura M;Ohkuma S
• 通讯作者: Ohkuma S

「アルコール依存症病態発現時の神経薬理学的機序」アルコール・医学医療の最前線別冊 医学のあゆみ(竹井謙之編集)

《酒精依赖病理发生时的神经药理学机制》酒精与医疗前沿医学史专卷（武井健行主编）

• 发表时间: 2008
• 作者: 伊勢文孝;他;桂 昌司
• 通讯作者: 桂 昌司

グルタミンによる消化管保護効果発現のメカニズム

谷氨酰胺胃肠道保护作用机制

• 发表时间: 2010
• 作者: 赤木玲子;等
• 通讯作者: 等

Direct Evidence for the Up-regulation of Vps34 Regulated by PKCγ During Short-Term Treatment With Morphine

• DOI: 10.1002/syn.20612
• 发表时间: 2009-05-01
• 期刊: SYNAPSE
• 影响因子: 2.3
• 作者: Shebasaki, Masahiro;Kurokawa, Kazuhiro;Ohkuma, Seitaro
• 通讯作者: Ohkuma, Seitaro

Molecular mechanism of antihypertensive azelnidipine-induced augmention of insulin sensitivity : Evidence for antioxidative effect of insulin target 3T3-L1 cells.

抗高血压阿折地平诱导胰岛素敏感性增强的分子机制：胰岛素靶标 3T3-L1 细胞抗氧化作用的证据。

• 发表时间: 2010
• 期刊: Diabetes 59
• 作者: Tatsumi F;Katsura M;Hashiramoto M;Tawaramoto K;Hamamoto S;Shimoda M;Kanda Y;Matsuda M;Kaku K
• 通讯作者: Kaku K