Gene expression profiling of bone-marrow derived diffuse large B-cell lymphoma

骨髓源性弥漫性大 B 细胞淋巴瘤的基因表达谱

• 批准号: 20590339
• 负责人: YAMASHITA Yoriko
• 金额: $ 1.5万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590339/
• 关键词: 骨髄; びまん性大細胞型B細胞性リンパ腫; 免疫グロブリン遺伝子; ケモカイン; ケモカインリセプター; マイクロアレイ; XCR1; B1細胞

A total of 29 cases of diffuse large B-cell lymphoma initially manifesting in the bone marrow (BM-DLBCL) were analyzed for V_H gene sequence, and expression microarray of chemokines and chemokine receptors and immunohistochemical analysis were done. Seminested polymerase chain reaction (PCR) and sequencing analyses of 18 cases revealed that V_H3-7, V_H4-34, and V_H4-39 were frequently used, and the V_H gene usage in 14 of 18 V_H genes including VH3-48, VH3-23, VH3-21 were those associated with autoimmune diseases. Furthermore, cDNA microarray analysis specific for chemokine and chemokine receptors revealed that chemokine receptor XCR1 expression was significantly elevated in the BM-DLBCL cases (P<.05), which was confirmed by quantitative reverse transcriptase-PCR and immunohistochemical analysis. Expression of the chemokine receptor XCR1 and frequent usage of autoreactive V_H genes seem to be distinct characteristics of BM-DLBCL. Am J Clin Pathol, In press.

对29例原发于骨髓的弥漫性大B细胞淋巴瘤（BM-DLBCL）进行VH基因测序、趋化因子及其受体表达谱芯片和免疫组化分析。半巢式聚合酶链反应（PCR）和测序分析表明，VH 3 -7、VH 4 -34和VH 4 -39是18例自身免疫性疾病中使用频率最高的3个基因，其中VH 3 -48、VH 3 -23、VH 3 -21等14个基因的VH基因使用与自身免疫性疾病有关。此外，特异性趋化因子和趋化因子受体的cDNA微阵列分析显示，趋化因子受体XCR 1在BM-DLBCL病例中的表达显著升高（P<0.05），这通过定量逆转录-PCR和免疫组化分析证实。趋化因子受体XCR 1的表达和自身反应性V_H基因的频繁使用可能是BM-DLBCL的显著特征。美国临床病理学杂志，出版中。

项目成果

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Biased Immunoglobulin VH Recombination and Increased Expression of Chemokine Receptor XCRl Are Distinct Features of Bone Marrow-Derived Diffuse Large B-Cell Lymphoma

偏向性免疫球蛋白 VH 重组和趋化因子受体 XCR1 表达增加是骨髓源性弥漫性大 B 细胞淋巴瘤的独特特征

• 发表时间: 2009
• 作者: Yamashita Y;et al.
• 通讯作者: et al.

A Novel Method for Efficient Collection of Normal Mesothelial Cells In Vivo

• DOI: 10.3164/jcbn.10-01
• 发表时间: 2010-05-01
• 期刊: JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION
• 影响因子: 2.4
• 作者: Jiang, Li;Yamashita, Yoriko;Toyokuni, Shinya
• 通讯作者: Toyokuni, Shinya

Omental flap closure of refractory wounds : rat model.

难治性伤口的大网膜瓣闭合：大鼠模型。

• 发表时间: 2010
• 期刊: Nagoya J Med Sci. 72(1-2)
• 作者: Hishida M;Toriyama K;Yamashita Y;Akatsuka S;Hayakawa A;Torii S;Kamei Y.
• 通讯作者: Kamei Y.

骨髄に初発するDLBCLは免疫グロブリンVHファミリーの偏りとケモカインリセプターXCR1の発現を特徴とする

最初发生在骨髓中的 DLBCL 的特征是免疫球蛋白 VH 家族的偏差和趋化因子受体 XCR1 的表达。

• 发表时间: 2009
• 作者: 山下依子、梶浦大;中村栄男、森尚義
• 通讯作者: 中村栄男、森尚義