Gene expression profiling of bone-marrow derived diffuse large B-cell lymphoma

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A total of 29 cases of diffuse large B-cell lymphoma initially manifesting in the bone marrow (BM-DLBCL) were analyzed for V_H gene sequence, and expression microarray of chemokines and chemokine receptors and immunohistochemical analysis were done. Seminested polymerase chain reaction (PCR) and sequencing analyses of 18 cases revealed that V_H3-7, V_H4-34, and V_H4-39 were frequently used, and the V_H gene usage in 14 of 18 V_H genes including VH3-48, VH3-23, VH3-21 were those associated with autoimmune diseases. Furthermore, cDNA microarray analysis specific for chemokine and chemokine receptors revealed that chemokine receptor XCR1 expression was significantly elevated in the BM-DLBCL cases (P<.05), which was confirmed by quantitative reverse transcriptase-PCR and immunohistochemical analysis. Expression of the chemokine receptor XCR1 and frequent usage of autoreactive V_H genes seem to be distinct characteristics of BM-DLBCL. Am J Clin Pathol, In press.