Verification of hypoxia-reoxygenation as one of an internal carcinogenic factor for epithelium

我研究了一系列缺血再灌注条件是否可以作为内源性致癌因子作用于正常上皮细胞。我使用了三种上皮细胞系来检查这些细胞系在一系列缺氧-复氧条件下培养后是否可以转化为致瘤细胞，即，O_2浓度为1%和20%的培养箱之间的差异。该细胞系在连续传代后，在裸鼠体内零星转化为致瘤细胞。我还用小鼠肾脏检测了一系列缺血再灌注条件是否致癌，到目前为止，我还没有在缺血再灌注器官中检测到任何肿瘤。我需要用另一个器官检查可能的转化。

I examined whether a series of ischemia-reperfusion condition could act on normal epithelial cells as an endogenous carcinogenic factor. I used three epithelial cell lines to examine whether those cell lines could be converted into tumorigenic ones after cultivation in a series of hypoxia-reoxygenation condition, i.e., between the incubators kept at 1% and 20% O_2 concentrations. The cell lines were sporadically converted into tumorigenic ones in nude mice after serial long passages. I also examined whether a series of ischemia-reperfusion condition could be carcinogenic by using mouse kidney ; so far I have not detected any tumors in the ischemia-reperfusion organ. I need to examine possible conversion using another organ.