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Morphological and molecular pathological study of mucin -depleted foci as the preneoplastic lesion for depressed flat type on colon carcinogenesis

粘蛋白缺失病灶作为凹陷扁平型结肠癌癌前病变的形态学和分子病理学研究

基本信息

批准号：
20590405
负责人：
YOSHIMI Naoki
金额：
$ 3万
依托单位：
University of the Ryukyus
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2008
资助国家：
日本
起止时间：
2008 至 2010
项目状态：
已结题

项目摘要

Mucin-depleted foci (MDF), formed by dysplastic crypts devoid of mucin production, have identified in the colon of carcinogen-treated rodents. MDF are correlated with colon carcinogenesis and seemed to serve as precancerous lesion of flat-depressed colorectal cancer (CRC). We have, therefore, searched for MDF in sporadic CRC patients in larger sample size to reveal the histological characteristics of human MDF. Methods : Colonic samples from 53 patients of sporadic CRC were stained with Alcian blue and examined the presence of MDF. In the same samples, we also examined the presence of ACF and noticed that some MDF have ACF-like appearance. We defined these lesions as "protruded MDF" and analyzed histological features of these colonic mucosal lesions (ACF, MDF and protruded MDF). Results : We found a total of 354, 41 and 19 colonic mucosal lesions with a mean multiplicity of 44, 38.9 and 66.9 crypts (ACF, MDF and protruded MDF, respectively). The density of MDF like lesions (MDF and protruded MDF) was 0.0082 lesions/cm^2. ACF from sporadic CRC patients were corresponding to hyperplastic or nondysplasic lesions. On the other hand, MDF and protruded MDF were corresponding to low grade of dysplasia. We also found that Paneth cell metaplasia and infiltration of inflammatory cells were specific feature of MDF like lesions. Conclusions : This is the first report that investigating density and histological features of MDF like lesions from sporadic CRC patients in large sample size. MDF and protruded MDF which having both MDF and ACF appearance were suggested to serve as more advanced preneoplastic lesions in human colorectal carcinogenesis.

黏液蛋白枯竭灶（MDF），由缺乏黏液蛋白产生的发育不良隐窝形成，已在致癌物治疗的啮齿动物的结肠中发现。MDF与结肠癌发生有关，似乎是扁平凹陷型结直肠癌（CRC）的癌前病变。因此，我们在更大样本量的散发性结直肠癌患者中寻找MDF，以揭示人类MDF的组织学特征。方法：对53例散发性结直肠癌患者的结肠标本进行阿利新蓝染色，检测MDF的存在。在相同的样本中，我们也检查了ACF的存在，并注意到一些MDF具有ACF样外观。我们将这些病变定义为“突出的MDF”，并分析了这些结肠粘膜病变的组织学特征（ACF、MDF和突出的MDF）。结果：共发现354例、41例和19例结肠粘膜病变，平均隐窝数分别为44、38.9和66.9个（ACF、MDF和突出MDF）。MDF样病变（MDF和突出MDF）的密度为0.0082个/cm^2。散发性结直肠癌患者的ACF对应于增生性或非增生性病变。另一方面，MDF和突出的MDF对应于低级别的不典型增生。我们还发现，Paneth细胞化生和炎症细胞浸润是MDF样病变的特异性特征。结论：这是第一篇大样本量研究散发性结直肠癌患者MDF样病变密度和组织学特征的报道。MDF和突出的MDF同时具有MDF和ACF的外观，被认为是人类结直肠癌发生中更晚期的肿瘤前病变。