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Molecular pathological study on tumorigenesis of beta-catenin gene alterated foci in rat colon mucosa

大鼠结肠黏膜β-catenin基因改变灶致瘤的分子病理学研究

基本信息

批准号：
13670208
负责人：
YOSHIMI Naoki
金额：
$ 2.11万
依托单位：
University of the Ryukyus
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

Although beta-catenin accumulated crypts(BCAC)is an useful biomarker in the retrieval of the chemopreventive agents on rat colon carcinogenesis, the detection of BCAC is complicated, since the preparation manufacture is needed.In this study, it was examined the mucin or mucoprotein in neoplastic lesions using the High-iron diamine alcian blue(HID+AB) PH2.5 and AB pH1.0 staining for dissolving these problems.Negative lesions by the HID+AB pH2.5 staining were significantly included in BCAC, further than the aberrant crypt foci(ACE), that is conventional lesions in rat colon carcinogenesis.Especially, the rate of concordance between negative lesion nest HID+AB pH2.5 staining and BCAC which consist of crypts over 4 was high.Therefore, the negative lesion, which consisted of crypts over 4, was able to seem to become the substitution of BCAC.However, the detection of BCAC in the proximal colon was difficult for the HID+AB pH2.5 staining because of the characteristics of the histological feature of proximal colon and the HLD+AB pH2.5 staining. Thus, we examined the possibility of the application of AB pH1.0 alone staining by which sulfomucin and sialomucin can be simultaneously represented as well as HJD+AB pH2.5 staining.The AB pH1.0 alone staining was able to detect the negative lesions easier than HID+AB pH2.5 staining, and it was also proven that the detection was possible on those in the proximal colon.In conclusion, both HID+AB pH2.5 staining and AB pH1.0 alone staining were detectable the conventional preneoplastic lesions such as BCAC and ACF.In the future, it is necessary to search the mutual relationship among these negative lesions, BCAC and ACF.

β -连环蛋白积累隐窝（-catenin accumulation crypts， BCAC）是一种有用的生物标志物，可用于大鼠结肠癌化学预防药物的检测，但由于BCAC的制备需要，其检测过程较为复杂。本研究采用高铁二胺阿利新蓝（HID+AB） PH2.5和AB pH1.0染色法检测肿瘤病变中的粘蛋白或粘蛋白，以解决这些问题。在BCAC中，HID+AB pH2.5染色阴性的病变比大鼠结肠癌的常规病变异常隐窝灶（ACE）更明显。特别是，阴性病变巢HID+AB pH2.5染色与由4个以上隐窝组成的BCAC的一致性率很高。因此，由超过4个隐窝组成的阴性病变似乎能够成为BCAC的替代品。然而，由于近端结肠的组织学特征和HLD+AB pH2.5染色的特点，HID+AB pH2.5染色较难检测近端结肠的BCAC。因此，我们研究了AB pH1.0单独染色的可能性，该染色可以同时表示磺胺和唾液蛋白，以及HJD+AB pH2.5染色。AB pH1.0单独染色比HID+AB pH2.5染色更容易检测到阴性病变，并且也证明了对近端结肠的检测是可能的。综上所述，HID+AB pH2.5染色和AB pH1.0单独染色均可检测到BCAC、ACF等常规瘤前病变。今后需要进一步研究这些阴性病变与BCAC、ACF之间的相互关系。