Molecular pathological study on tumorigenesis of beta-catenin gene alterated foci in rat colon mucosa

虽然β-连环蛋白累积隐窝(BCAC)是一种有用的生物标志物，可用于寻找预防大鼠结肠癌的化学预防药物，但BCAC的检测很复杂，因为需要制备制剂。在本研究中，用高铁二胺阿利新蓝(HID+AB)PH2.5和AB PH1.0染色检测了肿瘤病变中的粘蛋白或粘蛋白。HID+AB PH2.5染色的阴性病变显著包括BCAC，而不包括变异隐窝病灶(ACE)，即大鼠结肠癌的常规病变。尤其是，HID+AB PH2.5染色阴性病变与隐窝大于4个的BCAC符合率较高，隐窝大于4个的阴性病变可作为BCAC的替代物。HID+AB pH 2.5染色由于近端结肠的组织学特点和HLD+AB pH 2.5染色的特点，较难发现近端结肠的BCAC。因此，我们探讨了AB pH 1.0单独染色同时表达磺基粘蛋白和唾液粘蛋白以及HJD+AB pH 2.5染色的可能性。AB pH 1.0单独染色比HID+AB pH 2.5染色更容易发现阴性病变，也证明了在近端结肠的检测是可能的。结论：HID+AB pH 2.5染色和AB pH 1.0单独染色都可以检测到传统的癌前病变，如BCAC和ACF。

Although beta-catenin accumulated crypts(BCAC)is an useful biomarker in the retrieval of the chemopreventive agents on rat colon carcinogenesis, the detection of BCAC is complicated, since the preparation manufacture is needed.In this study, it was examined the mucin or mucoprotein in neoplastic lesions using the High-iron diamine alcian blue(HID+AB) PH2.5 and AB pH1.0 staining for dissolving these problems.Negative lesions by the HID+AB pH2.5 staining were significantly included in BCAC, further than the aberrant crypt foci(ACE), that is conventional lesions in rat colon carcinogenesis.Especially, the rate of concordance between negative lesion nest HID+AB pH2.5 staining and BCAC which consist of crypts over 4 was high.Therefore, the negative lesion, which consisted of crypts over 4, was able to seem to become the substitution of BCAC.However, the detection of BCAC in the proximal colon was difficult for the HID+AB pH2.5 staining because of the characteristics of the histological feature of proximal colon and the HLD+AB pH2.5 staining. Thus, we examined the possibility of the application of AB pH1.0 alone staining by which sulfomucin and sialomucin can be simultaneously represented as well as HJD+AB pH2.5 staining.The AB pH1.0 alone staining was able to detect the negative lesions easier than HID+AB pH2.5 staining, and it was also proven that the detection was possible on those in the proximal colon.In conclusion, both HID+AB pH2.5 staining and AB pH1.0 alone staining were detectable the conventional preneoplastic lesions such as BCAC and ACF.In the future, it is necessary to search the mutual relationship among these negative lesions, BCAC and ACF.