Aminophylline induced-neuronal cell injury under hypoxic conditions and the survey of the protective agents against the injury.

氨茶碱是一种亲水性的甲基黄嘌呤衍生物，在日本经常用于治疗早产儿呼吸暂停患者，以防止进一步的呼吸损害。结果表明，在低潮气量机械通气所致的低氧条件下，服用推荐剂量的氨茶碱可加重豚鼠的神经元损伤，表现为血清CK-BB活性升高，海马CA1区细胞数量减少，脑组织脂质过氧化水平升高，GSH/GSSG比值降低。我们还表明，用自由基清除剂依达拉奉治疗可以减弱这些影响。结果提示，氨茶碱治疗可加重低氧血症患者的中枢神经系统损害。

Aminophylline, a hydrophilic methylxanthine derivative, is often used to treat patients with apnea of prematurity to prevent further respiratory impairment in Japan. We showed that aminophylline, used at recommended doses, aggravated neuronal injury as indicated by increased serum CK-BB activity, a reduction in the number of cells in the hippocampal CA1 region, and increased lipid peroxide levels and a decreased GSH:GSSG ratio in brain tissue under hypoxic conditions induced by low-tidal volume ventilation in guinea pigs. We also showed that these effects were attenuated by treatment with the free radical scavenger, edaravone. The results suggest that aminophylline treatment aggravates the central nervous system of patients with hypoxemia.