Neurobehavioral changes in metallothionein-null mice postnatal exposed to low levels mercury vapor.

出生后暴露于低水平汞蒸气的金属硫蛋白缺失小鼠的神经行为变化。

• 批准号: 20590611
• 负责人: YOSHIDA Minoru
• 金额: $ 3.08万
• 依托单位: Hachinohe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590611/
• 关键词: 神経行動毒性; 水銀蒸気曝露; 新生児; メタロチオネイン; マイクロアレイ; 無機水銀; 水銀蒸気; マウス; 脳内水銀濃度; 加齢

Metallothionein MT-null and wild-type mice were repeatedly exposed to Hg^0 at 0.057 mg/m^3, which were similar to the current threshold value (TLV), until the 20th of postpartum. Hg^0-exposed mice of both strains did not show behavioral changes such as total locomotor activity, learning ability and spatial learning ability. However, Hg^0 exposure resulted in significant alterations in gene expression in the brain of both strains using DNA microarray analysis. A number of altered genes in MT-null mice were more than those in wild-type mice. When the exposure levels elevated to 0.198mg/m^3, MT-null mice showed a significant decrease in total locomotor activity in female, though learning ability and spatial learning ability were not affected. A number of altered genes were further increased by elevation of Hg^0 exposure levels. DNA microarray analysis may provide useful information to elucidate development of behavioral toxicity following a low-level exposure to Hg^0.

将MT基因敲除小鼠和野生型小鼠反复暴露于0.057 mg/m^3的汞（与当前阈值（TLV）相似），直至产后20天。两种品系的小鼠均未表现出行为学改变，如总的自发活动、学习能力和空间学习能力。然而，使用DNA微阵列分析，Hg^0暴露导致两种品系大脑中基因表达的显著改变。MT基因敲除小鼠的突变基因数明显多于野生型小鼠。当暴露水平增加到0.198mg/m^3时，MT基因敲除小鼠的总活动能力显著降低，但学习能力和空间学习能力不受影响。随着汞暴露水平的升高，许多改变的基因进一步增加。DNA微阵列分析可能为阐明低水平汞暴露后行为毒性的发展提供有用的信息。

项目成果

Emergence of delayed methylmercury toxicity after perinatal exposure in metallothionein-null and wild-type C57BL mice.

• DOI: 10.1289/ehp.10906
• 发表时间: 2008-06
• 期刊: ENVIRONMENTAL HEALTH PERSPECTIVES
• 影响因子: 10.4
• 作者: Yoshida, Minoru;Shimizu, Natsuki;Suzuki, Megumi;Watanabe, Chiho;Satoh, Masahiko;Mori, Kouki;Yasutake, Akira
• 通讯作者: Yasutake, Akira

小規模金鉱山における水銀による作用業者の健康状況について～文献検索から～

关于小型金矿受汞影响的工人健康状况～来自文献检索～

• 发表时间: 2009
• 作者: 吉田稔;村田勝敬;佐藤洋
• 通讯作者: 佐藤洋

Neurotoxic action of inorganic mercury injected in the intraventricular space of mouse cerebrum.

注射到小鼠大脑室内空间的无机汞的神经毒性作用。

• 发表时间: 2010
• 期刊: J Toxicol Sci. 35(5)
• 作者: Yasutake A;Marumoto M;Yoshida M.
• 通讯作者: Yoshida M.

マウスにおける無機水銀の神経毒性作用 : 無機水銀脳室内投与モデルを用いた検討

无机汞对小鼠的神经毒性作用：使用无机汞脑室内给药模型的研究

• 发表时间: 2009
• 作者: 吉田 稔;安武 章
• 通讯作者: 安武 章

Contribution of NIMD to Mercury Research in Guizhou, China, II. Human Exposure Research : High Exposure of Chinese Mercury Mine Workers to Mercury Vapor and Increased Methylmercury Levels in the Hair

NIMD 对中国贵州汞研究的贡献 II．

• 发表时间: 2009
• 作者: Mineshi Sakamoto;Xinbin Feng;Minoru Yoshida;Katsuyuki Murata;Akira Yasutake
• 通讯作者: Akira Yasutake