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Modulatory Effects of Vascular Humoral Factors on Membrane Function in Metabolic Syndrome

血管体液因子对代谢综合征膜功能的调节作用

基本信息

批准号：
20590710
负责人：
TSUDA Kazushi
金额：
$ 2.91万
依托单位：
Kansai College of Oriental Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2008
资助国家：
日本
起止时间：
2008 至 2010
项目状态：
已结题

项目摘要

It has been shown that abnormalities in physical properties of the cell membranes may underlie the defects that are strongly linked to hypertension, stroke, and other cardiovascular diseases. In the present study, we examined the membrane fluidity (a reciprocal value of membrane microviscosity) of erythrocytes in hypertensive subjects with metabolic disorders by using an electron spin resonance (ESR) method. We demonstrated that membrane fluidity of erythrocytes was significantly lower in hypertensive subjects than in normotensive subjects. It was shown that the lower was the plasma nitric oxide (NO) metabolite level, the lower was the membrane fluidity of erythrocytes. In contrast, the higher was the plasma asymmetric dimethylarginine (ADMA) level, the lower was the membrane fluidity of erythrocytes. Plasma homocysteine concentration was significantly increased in hypertensive subjects compared with normotensive subjects. The reduced membrane fluidity of erythrocytes was associated with higher plasma homocysteine levels. In an in vitro study, the selective estrogen receptor modulator (SERM), tamoxifen, significantly restored membrane fluidity of erythrocytes in elderly women, which was partially mediated by the NO-dependent mechanism. We propose that reduced membrane fluidity of erythrocytes might cause a disturbance in the blood rheologic behavior and the microcirculation, and that improvement of membrane function could contribute, at least in part, to the prevention against circulatory disorders in subjects with hypertension and the metabolic syndrome.

研究表明，细胞膜物理特性的异常可能是与高血压、中风和其他心血管疾病密切相关的缺陷的基础。在本研究中，我们用电子自旋共振（ESR）方法检测了高血压代谢紊乱患者红细胞的膜流动性（膜微粘度的倒数值）。我们发现高血压患者的红细胞膜流动性明显低于正常血压患者。结果表明，血浆一氧化氮（NO）代谢物水平越低，红细胞膜流动性越低。血浆不对称二甲基精氨酸（ADMA）水平越高，红细胞膜流动性越低。高血压患者血浆同型半胱氨酸浓度明显高于正常者。红细胞膜流动性降低与血浆同型半胱氨酸水平升高有关。在一项体外研究中，选择性雌激素受体调节剂（SERM）他莫昔芬（tamoxifen）可显著恢复老年妇女红细胞的膜流动性，这部分是由no依赖机制介导的。我们认为，红细胞膜流动性的降低可能会引起血液流变学行为和微循环的紊乱，而膜功能的改善至少在一定程度上有助于预防高血压和代谢综合征患者的循环障碍。