The effect of aging and smoking on the phagocytosis of apoptotic cells in alveolar macrophages

衰老和吸烟对肺泡巨噬细胞凋亡细胞吞噬作用的影响

• 批准号: 20590890
• 负责人: NASUHARA Yasuyuki
• 金额: $ 2.91万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590890/
• 关键词: 閉塞性肺疾患; マクロファージ; 喫煙; アポトーシス; 炎症; マクロフィージ

Our aims of this study are to examine expressions of various receptors for recognition and/or clearance of apoptotic cells on macrophages and assess the relationship with aging and/or smoking. The expression of phosphatidylserine receptor (PSR) and scavenger receptor A (SR-A) in alveolar macrophages was downregulated in smokers when compared to lifelong non-smokers only in the older subjects, but not in younger subjects. The expression of PSR is elevated on U937-derived macrophage like cells if these are incubated in CSE. The cigarette smoke extract-induced upregulation of PSR was inhibited by the vascular endothelial growth factor (VEGF) receptor blockade, SU5614, in U937-derived macrophage-like cells. These results showed that VEGF was indirectly associated with the ability to phagocytose apoptotic cells through the expression of PSR.

本研究的目的是检测巨噬细胞上识别和/或清除凋亡细胞的各种受体的表达，并评估与衰老和/或吸烟的关系。与终身不吸烟者相比，吸烟者肺泡巨噬细胞中磷脂酰丝氨酸受体（PSR）和清道夫受体A（SR-A）的表达仅在老年受试者中下调，而在年轻受试者中则没有。如果将U937衍生的巨噬细胞样细胞在CSE中孵育，则PSR的表达在这些细胞上升高。在U937衍生的巨噬细胞样细胞中，香烟烟雾提取物诱导的PSR上调被血管内皮生长因子（VEGF）受体阻断剂SU 5614抑制。这些结果表明，VEGF通过表达PSR与吞噬凋亡细胞的能力间接相关。

项目成果

COPDの気流制限に及ぼす3次元CT画像で評価した気腫病変と気道病変の関与

3D CT 图像评估肺气肿病变和气道病变对 COPD 气流受限的影响

• 发表时间: 2008
• 作者: 長谷川 大;南須原康行;牧田比呂仁;別役智子;西村正治
• 通讯作者: 西村正治

アレルギー性気道炎症モデルにおけるDHMEQの効果

DHMEQ对过敏性气道炎症模型的影响

• 发表时间: 2011
• 作者: Shimizu K;et al.;清水健一
• 通讯作者: 清水健一

Duration of cigarette smoke (CS) exposure affects the resolution of lung inflammation after cessation in mice.

小鼠吸烟（CS）暴露的持续时间会影响戒烟后肺部炎症的消退。

• 发表时间: 2009
• 作者: Odajima N;Betsuyaku T;Inomata T;Yoshida T;Moriyama C;Suzuki K;Nagai K;Nasuhara Y;Nishimura M.
• 通讯作者: Nishimura M.

Relationship between improved airflow limitation and changes in airway calibre induced by inhaled anticholinergic agents in COPD

• DOI: 10.1136/thx.2008.103671
• 发表时间: 2009-04-01
• 期刊: THORAX
• 影响因子: 10
• 作者: Hasegawa, M.;Makita, H.;Nishimura, M.
• 通讯作者: Nishimura, M.

Airflow limitation and airway dimensions assessed per bronchial generation in older asthmatics.

对老年哮喘患者每代支气管的气流受限和气道尺寸进行评估。

• 发表时间: 2010
• 期刊: Respir Med. 104
• 作者: Shimizu K;Hasegawa M;Makita H;Nasuhara Y;Konno S;Nishimura M.
• 通讯作者: Nishimura M.