Mechanism of transcriptional regulation in cyanobacteria upon acclimation to high-light conditions

蓝藻适应高光条件时的转录调控机制

• 批准号: 20770026
• 负责人: HIHARA Yukako
• 金额: $ 2.66万
• 依托单位: Saitama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20770026/
• 关键词: シグナル伝達; 転写制御; シアノバクテリア; 光合成; 光順化

Previously, we analyzed the promoter architecture of the psaAB genes encoding reaction center subunits of photosystem I (PSI) in the cyanobacterium Synechocystis sp. PCC 6803. There exist two promoters, P1 and P2, both of which show typical high-light (HL) response of PSI genes ; their activities are high under low-light (LL) conditions but rapidly downregulated upon the shift to HL conditions. In this study, it was shown that a response regulator RpaB binds to multiple high-light regulatory 1 (HLR1) sequences in the upstream region of the psaAB genes. We explored the regulatory role of cis-elements, including these HLR1 sequences on the individual activity of P1 and P2. Our results suggest that cis-elements, including multiple HLR1 sequences, differently regulate the activities of dual promoters of the psaAB genes to achieve the fine-tuning of the gene expression.

以前，我们分析了启动子结构的psaAB基因编码的反应中心亚基的光系统I（PSI）的蓝藻集胞藻属PCC 6803。有两个启动子，P1和P2，这两个都表现出典型的高光（HL）响应的PSI基因，他们的活动是高的低光（LL）条件下，但迅速下调后，转移到HL条件。在这项研究中，它表明，反应调节RpaB结合到多个高光调节1（HLR1）序列的上游区域的psaAB基因。我们探讨了顺式元件（包括这些HLR1序列）对P1和P2各自活性的调节作用。我们的研究结果表明，顺式元件，包括多个HLR1序列，不同地调节psaAB基因的双启动子的活动，以实现基因表达的微调。

项目成果

Role of multiple HLR1 sequences in the regulation of the dual promoters of the psaAB genes in Synechocystis sp.

集胞藻中多个 HLR1 序列在 psaAB 基因双启动子调节中的作用。

• 发表时间: 2010
• 期刊: PCC 6803. Journal of Bacteriology 192
• 作者: Takahashi T;Nakai N;Muramatsu M;Hihara Y
• 通讯作者: Hihara Y

The response regulator RpaB binds to the upstream element of photosystem I genes to work for positive regulation under low-light conditions in Synechocystis sp.PCC 6803.

反应调节器 RpaB 与光系统 I 基因的上游元件结合，在集胞藻 PCC 6803 的弱光条件下发挥正向调节作用。

• 发表时间: 2009
• 期刊: Journal of Bacteriology 191
• 作者: Seino Y;Takahashi T;Hihara Y
• 通讯作者: Hihara Y

Transcriptional regulators working on environmental responses in Cyanobacteria

研究蓝藻环境反应的转录调节因子

• 发表时间: 2009
• 作者: Cintia G.Kawashima;Colette A.Matthewman;Siqi Huang;Bok-Rye Lee;Naoko Yoshimoto;Anna Koprivova;Ignacio Rubio-Somoza;Marco Todesco;Tina Rathjen;Kazuki Saito;Hideki Takahashi;Tamas Dalmay;Stanislav Kopriva;Cintia G.Kawashima;Hihara Y
• 通讯作者: Hihara Y

Difference in metabolite levels between photoautotrophic and photomixotrophic cultures of Synechocystis sp. PCC 6803 examined by capillary electrophoresis electrospray ionization mass spectrometry.

• DOI: 10.1093/jxb/ern157
• 发表时间: 2008
• 期刊: Journal of experimental botany
• 影响因子: 6.9
• 作者: Takahashi H;Uchimiya H;Hihara Y
• 通讯作者: Hihara Y

An AbrB-like transcriptional regulator, SI10822, is essential for the activation of nitronen-renulated cenes in Svnechocvstis sp. PCC 6803

类似 AbrB 的转录调节因子 SI10822 对于 Svnechocvstis sp 中硝基烯化烯的激活至关重要。

• 发表时间: 2008
• 期刊: Plant Physiology 148
• 作者: Ishii A;Hihara Y
• 通讯作者: Hihara Y