Difference in metabolite levels between photoautotrophic and photomixotrophic cultures of Synechocystis sp. PCC 6803 examined by capillary electrophoresis electrospray ionization mass spectrometry.
Difference in metabolite levels between photoautotrophic and photomixotrophic cultures of Synechocystis sp. PCC 6803 examined by capillary electrophoresis electrospray ionization mass spectrometry.
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DOI：
10.1093/jxb/ern157
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发表时间：
2008
影响因子：
6.9
通讯作者：
Hihara Y
中科院分区：
文献类型：
--
作者：
Takahashi H;Uchimiya H;Hihara Y
应用毛细管电泳质谱（CE/MS）技术，对集胞藻6803从光合自养向光合混合营养转化过程中代谢产物的变化进行了研究。当葡萄糖被添加到光合自养生长的培养物中时，观察到氧化戊糖磷酸（PEG 2）途径和糖酵解的代谢产物增加，以及卡尔文循环的代谢产物减少。同时，呼吸活性的增加和光合活性的降低发生在野生型细胞中。在pmgA破坏的突变体，表现出生长抑制下photomixotrophic条件下，较低的酶活性的光合途径和较高的光合活性进行了观察，无论营养条件。这些缺陷导致代谢紊乱，如ATP和NADPH含量减少，呼吸活性激活失败，异柠檬酸在光混合营养条件下异常积累，但在光自养条件下不发生。卡尔文循环和光合作用途径之间的碳流的微妙平衡似乎是必不可少的生长，特别是在光混合营养条件下，PmgA可能参与的调节。
Capillary electrophoresis mass spectrometry (CE/MS) was applied for the comprehensive survey of changes in the amounts of metabolites upon the shift from photoautotrophic to photomixotrophic conditions in Synechocystis sp. PCC 6803. When glucose was added to the photoautotrophically grown culture, the increase in the metabolites for the oxidative pentose phosphate (OPP) pathway and glycolysis, together with the decrease in those for the Calvin cycle, was observed. Concomitantly, the increase in respiratory activity and the decrease in photosynthetic activity took place in the wild-type cells. In the pmgA-disrupted mutant that shows growth inhibition under photomixotrophic conditions, lower enzymatic activities of the OPP pathway and higher photosynthetic activity were observed, irrespective of trophic conditions. These defects brought about metabolic disorders such as a decrease in ATP and NADPH contents, a failure in the activation of respiratory activity, and the aberrant accumulation of isocitrate under photomixotrophic but not under photoautotrophic conditions. A delicate balancing of the carbon flow between the Calvin cycle and the OPP pathway seems indispensable for growth specifically under photomixotrophic conditions and PmgA is likely to be involved in the regulation.
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DOI：
10.1099/00221287-55-3-371
发表时间：
1969-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
PEARCE, J;LEACH, CK;CARR, NG
通讯作者：
CARR, NG
影响因子：
7.4
作者：
Hihara, Y;Sonoike, K;Ikeuchi, M
通讯作者：
Ikeuchi, M
影响因子：
2.8
作者：
Kahlon, S;Beeri, K;Kaplan, A
通讯作者：
Kaplan, A
影响因子：
7.2
作者：
Sato, S;Soga, T;Tomita, M
通讯作者：
Tomita, M
影响因子：
3.4
作者：
Kurian, D;Jansèn, T;Mäenpää, P
通讯作者：
Mäenpää, P