regulation of acidic organelle localization by V-ATPase

V-ATP酶调节酸性细胞器定位

• 批准号: 20770090
• 负责人: GOTO Naomi
• 金额: $ 2.75万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20770090/
• 关键词: V-ATPase; 小胞分泌; 酸性オルガネラ; リソソーム; 小胞輸送; 酸性環境; オルガネラ; 破骨細胞

Vacuolar type ATPase (V-ATPase) consists of 13 different subunits, and transports protons using energy from ATP hydrolysis. V-ATPase has variety of subunit isoforms, consistent with the different pH in acidic compartment. Previously, we showed that a3, one of 'a' subunit isoforms, was induced during osteoclast differentiation. In this study, we found the induction of d2 isoform and its interaction with a3 in differentiated cells. This interaction suggests that osteoclasts have a novel combination of V-ATPase isoforms.

液泡型ATPase（V-ATPase）由13个不同的亚基组成，并使用ATP水解中的能量运输质子。 V-ATPase具有多种亚基同工型，与酸性室中不同的pH值一致。以前，我们表明在破骨细胞分化过程中诱导了A3，一种“ A”亚基同工型。在这项研究中，我们发现D2同工型的诱导及其与分化细胞中A3的相互作用。这种相互作用表明破骨细胞具有新颖的V-ATPase同工型的组合。

项目成果

Dynamic and functional assembly of the AAA peroxins, Pexlp and Pex6p, and their membrane receptor Pex26p involved in shuttling of the PTS1 receptor Pex5n in peroxisome biogenesis

AAA过氧化物酶、Pexlp和Pex6p及其膜受体Pex26p的动态和功能组装参与过氧化物酶体生物发生中PTS1受体Pex5n的穿梭

• 发表时间: 2008
• 期刊: Biochemical Society transactions 36
• 作者: Yukio Fujiki;Non Miyata;Naomi Matsumoto;Shigehiko Tamura
• 通讯作者: Shigehiko Tamura

Characterization of organelles with V-ATPase a3 subunit in osteoclasts

破骨细胞中 V-ATPase a3 亚基细胞器的表征

• 发表时间: 2009
• 作者: 齊藤泰之、的崎尚;他;後藤(松元)奈緒美
• 通讯作者: 後藤(松元)奈緒美