Analysis of the molecular mechanism of the quantitative resistance to grapevine anthracnose.

通过改变分生孢子的浓度，建立了一种生产足量分生孢子的可靠方法。它使我们能够方便、稳定地评估葡萄对炭疽病的抗性。对94个葡萄品种进行了抗性鉴定，并用病斑大小有效地反映了各葡萄品种的抗性。抗病葡萄在真菌入侵后不久即可观察到过敏反应和棕色化合物(酚类)的积累，限制了对植物细胞的进一步入侵。这表明，中抗葡萄品种可能具有基本相同的抗毒机理。

A reliable method to produce sufficient amount of conidia of Elsinoe ampelina was developed by modifying the concentration of the media. It enables us to evaluate the resistance of grapevines to anthracnose easily and stably. The resistance of 94 grapevines was evaluated, and the resistance of each vine was effectively shown by their lesion sizes. The hypersensitive response and the accumulation of brown compounds (phenolics) were observed soon after the invasion of the fungus in the resistant grapevine, and the further invasion into the plant cells was restricted. It was implied that the moderate resistant Vitis vinifera cultivars might have almost same mechanism against E. ampelina.