Molecular dissection of 35S promoter-specific DNA methylation in gentian.

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This study is intended to determine a causal factor for 35S promoter sequence-specific silencing in gentian. As a consequence of the analysis of nuclear factors in gentian, we found two regions in the 35S promoter that showed specific binding to nuclear factors of gentian. These two regions were consistent with the highly de novo methylated regions in the transgenic gentian plants. Since the two regions share common motifs, the gentian nuclear factors may involve the de novo methylation of the two regions.