Investigation into the molecular mechanisms of reproductive manipulation of host insect by endosymbiotic bacteria.

内共生细菌调控宿主昆虫生殖的分子机制研究。

• 批准号: 20780042
• 负责人: ANBUTSU Hisashi
• 金额: $ 2.66万
• 依托单位: National Institute of Advanced Industrial Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20780042/
• 关键词: 共生細菌; ショウジョウバエ; スピロプラズマ; ファージ; 生殖操作; 雄殺し; 免疫機構; 性比; P58遺伝子ファミリー; 抗菌タンパク質; 分子機構

This study has been conducted using the endosymbiotic system consists of host insects, Drosophila fruit flies, and symbiotic bacteria, Spiroplasma, which cause "male killing" on its hosts. From the three Spiroplasma strains, genome sequences of their phages were identified and characterized. It was shown that Spiroplasma infections neither induced nor suppressed the systemic immune response of the host. In addition, it was also determined that the male-killing Spiroplasma is capable of suppressing the constitutive immune response and is resistant to mounted immune attacks. Sixteen insertion lines, which showed extremely male-biased sex ratio, have been obtained and the possible causative genes have been identified.

本研究使用由宿主昆虫果蝇和共生细菌螺原体组成的内共生系统进行，螺原体对宿主造成“雄性杀伤”。从三个螺原体菌株中，鉴定并表征了它们的基因组序列。结果表明，螺原体感染既不诱导也不抑制宿主的全身免疫应答。此外，还确定了雄性杀伤螺原体能够抑制组成性免疫应答，并对安装的免疫攻击具有抗性。获得了16个性比极度偏向雄性的插入系，并鉴定了可能的致病基因。

项目成果

ショウジョウバエ・スピロプラズマ共生系を用いて昆虫内部共生のメカニズムを探る

利用果蝇-螺原体共生系统探索昆虫内共生机制

• 发表时间: 2009
• 作者: 安佛尚志;深津武馬;安佛尚志・深津武馬;安佛尚志
• 通讯作者: 安佛尚志

Infection dynamics and immune resistance of male-killing and non-male-killing spiroplasmas

雄性致死性和非雄性致死性螺原体的感染动态和免疫抵抗力

• 发表时间: 2010
• 作者: 安佛尚志;深津武馬
• 通讯作者: 深津武馬

なぜ共生細菌スピロプラズマは宿主の免疫システムによって排除されないのか

为什么共生细菌螺原体没有被宿主的免疫系统消灭？

• 发表时间: 2009
• 作者: 安佛尚志;深津武馬;安佛尚志・深津武馬;安佛尚志;安佛尚志
• 通讯作者: 安佛尚志

Evasion, suppression and tolerance of Drosophila innate immunity by a male-killing Spiroplasma endosymbiont

• DOI: 10.1111/j.1365-2583.2010.01008.x
• 发表时间: 2010-08-01
• 期刊: INSECT MOLECULAR BIOLOGY
• 影响因子: 2.6
• 作者: Anbutsu, H.;Fukatsu, T.
• 通讯作者: Fukatsu, T.

Evasion, suppression and tolerance of Drosophila innate immuuity by a male-killing Spiroplasma endosymbiont

雄性杀伤螺原体内共生体对果蝇先天免疫的逃避、抑制和耐受

• 发表时间: 2010
• 期刊: Insect Molecular Biology
• 影响因子: 2.6
• 作者: 安佛尚志;深津武馬;安佛尚志・深津武馬
• 通讯作者: 安佛尚志・深津武馬