Suppressive mechanisms of action of polyphenols in cytokine-induced adipocyte dysfunction

在这项研究中，我们研究了多酚对细胞因子诱导的脂肪细胞功能障碍的分子功能。将3T3-L1脂肪细胞完全分化，并在几种多酚处理后用TNF-α或LPS处理，并测量培养基中的IL-6浓度。 TNF-α 增加脂肪细胞中 IL-6 的分泌，姜黄素抑制 IL-6 浓度。姜黄素抑制脂多糖诱导的一氧化氮水平。这些结果表明类姜黄素可能对细胞因子介导的脂肪细胞功能障碍具有抑制作用。现在，我们尝试进行微阵列实验，并检查基因表达谱。

In this study, we investigated molecular functions of polyphenols on cytokine-induced dysfunction of adipocytes. 3T3-L1 adipocytes were fully differentiated and treated with TNF-α or LPS after treatment of several polyphenols, and IL-6 concentration in the media was measured. TNF-α increased IL-6 secretion in adipocytes, and curcuminoids suppressed IL-6 concentration. Curcuminoids suppressed LPS-induced nitric oxide levels. These results suggest that curcuminoids may have suppressive effects on cytokine-mediated dysfunction of adipocytes. Now, we try to perform microarray experiments, and examine the gene expression profiles.