Determination of paternally methylated imprinted regions in mouse genome

小鼠基因组中父系甲基化印记区域的测定

• 批准号: 20780201
• 负责人: KAWAHARA Manabu
• 金额: $ 2.66万
• 依托单位: Saga University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20780201/
• 关键词: ゲノムインプリンティング; 核移植; バイオインフォマティクス; 胎盤形成; マイクロアレイ; 哺乳類; 卵子ゲノム

Parental genome functions in ontogeny are determined by interactions among transcripts from the maternal and paternal genomes, which contain many genes whose expression is strictly dependent on their parental origin as a result of genomic imprinting. Comprehensive recognition of the interactions between parental genomes is important for understanding genomic imprinting in mammalian development. The bi-maternal embryos were produced by the construction of oocytes from fully grown oocytes and nongrowing oocytes that contain double deletions in the H19 differentially methylated region (DMR) and the Dlk1-Dio3 intergenic germline-derived DMR. Through a sequence of studies, we demonstrated that analyses of ng/fg bi-maternal conceptuses helped in elucidating the roles of paternally methylated imprinted genes in mouse ontogeny.

亲本基因组在个体发育中的功能取决于母体和父亲基因组的转录本之间的相互作用，这些基因组包含许多基因，其表达严格取决于其父母的起源，这是基因组印记的结果。对父母基因组之间相互作用的全面认识对于理解哺乳动物发育中的基因组印记很重要。双血胚是由完全生长的卵母细胞和非卵母细胞构造的，这些卵母细胞在H19中包含双重缺失（DMR）和DLK1-DIO3基因间生殖线的DMR中包含双重缺失。通过一系列研究，我们证明了NG/FG双等概念的分析有助于阐明了小鼠本体发育中的亲属甲基化基因的作用。

项目成果

Defining contributions of paternally methylated imprinted genes at the IGF2-H19 and DLK1-GT12 domains to mouse placentation by transcriptomic analysis

通过转录组分析确定 IGF2-H19 和 DLK1-GT12 结构域的父系甲基化印记基因对小鼠胎盘的贡献

• 发表时间: 2009
• 期刊: The Journal of Biological Chemistry (In press)
• 作者: Manabu Kawahara;Shinnosuke Morita;Nozomi Takahashi;Tomohiro Kono
• 通讯作者: Tomohiro Kono

精子無し"マウスの作り方. 日本動物学会九州支部

日本动物学会九州分会如何制作无精子小鼠。

• 发表时间: 2008
• 作者: 川原学;森田慎之助;高橋望;河野友宏;川原学
• 通讯作者: 川原学

Involvement of Insulin-like Growth Factor 2 in Angiogenic Factor Transcription in Bi-maternal Mouse Conceptuses

• DOI: 10.1262/jrd.09-140a
• 发表时间: 2010-02-01
• 期刊: JOURNAL OF REPRODUCTION AND DEVELOPMENT
• 影响因子: 1.8
• 作者: Kawahara, Manabu;Wu, Qiong;Kono, Tomohiro
• 通讯作者: Kono, Tomohiro

Acetylation Level of Histone H3 in Early Embryonic Stages Affects Subsequent Development of Miniature Pig Somatic Cell Nuclear Transfer Embryos

• DOI: 10.1262/jrd.20245
• 发表时间: 2009-12-01
• 期刊: JOURNAL OF REPRODUCTION AND DEVELOPMENT
• 影响因子: 1.8
• 作者: Yamanaka, Ken-ichi;Sugimura, Satoshi;Sato, Eimei
• 通讯作者: Sato, Eimei

Defining Contributions of Paternally Methylated Imprinted Genes at the Igf2-H19 and Dlk1-Gtl2 Domains to Mouse Placentation by Transcriptomic Analysis

• DOI: 10.1074/jbc.m109.000299
• 发表时间: 2009-06-26
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Kawahara, Manabu;Morita, Shinnosuke;Kono, Tomohiro
• 通讯作者: Kono, Tomohiro