Study for accelerating effect of brain-derived neurotrophic factor on methylmercury-induced neuronal death

脑源性神经营养因子对甲基汞所致神经元死亡加速作用的研究

• 批准号: 20780221
• 负责人: SAKAUE Motohara
• 金额: $ 2.75万
• 依托单位: Azabu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20780221/
• 关键词: 脳由来神経影響因子; 神経細胞死; 小脳顆粒細胞; TrkB; 初代培養系; 神経芽細胞腫由来B35細胞; ラット; 脳由来神経栄養因子; 小脳粒細胞; 神経細胞腫由来B35細胞

Brain-derived neurotrophic factor (BDNF) is a maintenance protein for neuronal function and survive, but also stimulate methylmercury-induced neuronal cell death. In the present study, to expose mechanisms of the BDNF stimulation, we investigate using rat cerebellar granular cell culture system. Neurotrophin-4, another TrkB ligand, stimulated methylmercury-induced cell death. The stimulating effect of BDNF on the cell death was inhibited by treatment of anti-BDNF antibody that prevents BDNF from binding to TrkB. The results indicated that the stimulation by BDNF needs the BDNF binding to TrkB, which suggested BDNF/TrkB play as a sensitivity determiant factor.

脑源性神经营养因子（BDNF）是一种维持神经元功能和生存的蛋白质，同时也刺激甲基汞诱导的神经元细胞死亡。在本研究中，为了揭示 BDNF 刺激的机制，我们使用大鼠小脑颗粒细胞培养系统进行研究。 Neurotropin-4 是另一种 TrkB 配体，可刺激甲基汞诱导的细胞死亡。 BDNF对细胞死亡的刺激作用被抗BDNF抗体的处理所抑制，该抗体阻止BDNF与TrkB结合。结果表明BDNF的刺激需要BDNF与TrkB结合，这表明BDNF/TrkB作为敏感性决定因素。

项目成果

Acceleration of methylmercury-induced cell death of rat cerebellar neurons by brain-derived neurotrophic factor in vitro.

体外脑源性神经营养因子加速甲基汞诱导的大鼠小脑神经元细胞死亡。

• 发表时间: 2009
• 期刊: Brain Res. 1273
• 作者: Sakaue M;Mori N;Makita M;Fujishima K;Hara S;Arishima K;Yamamoto M.
• 通讯作者: Yamamoto M.

Acceleration of methylmercury-induced cell death of rat cerebellar neurons by brain-derived neurotrophic factor in vitro

脑源性神经营养因子体外加速甲基汞诱导的大鼠小脑神经元细胞死亡

• 发表时间: 2009
• 期刊: Brain Research (印刷中)
• 作者: Sakaue M.;et.al.
• 通讯作者: et.al.