Development of a Sequence-specific detection probe of damaged nucleobase for noninvasive DNA damage imaging.

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To develop a sequence-specific detection method of damaged nucleobase for noninvasive DNA damage imaging, we successfully created a novel bridged nucleic acid monomer that can recognize 8-hydroxyguanine in double-stranded DNA in a sequence-specific manner. Additionally, several nucleic acids, which convert into an oxidative lesion 5-formyluridne in double-stranded DNA in response to photo irradiation, were also synthesized. Moreover, we designed and synthesized a triplex forming oligonucleotide probe by organizing the 8-hydroxyguanine-recognizing nucleic acid monomer and acid-labile phosphoramidite linkage, and the fluorescent labeling of the probe was achieved for its noninvasive imaging use.