The mechanism for endothelial cell-specific gene expression through the Robo4 promoter.

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To investigate the mechanism for endothelial cell-specific gene expression, the function of regulatory elements in the ROBO4 promoter were analyzed using transgenic mice. The results indicated that both regulatory elements (REn and ETS) in the upstream and proximal promoter region were contributed to the promoter activity, and that 300bp proximal promoter region was responsible for endothelial cell-specific gene expression. In addition, the study on adenovirus vector containing ROBO4 promoter-luciferase sequence demonstrated that the ROBO4 promoter maintained endothelial cell -specificity in adenovirus.