Analysis of cytotoxic effect of sialic binding lectin (SBL)on human breast tumor cells

唾液酸结合凝集素（SBL）对人乳腺肿瘤细胞的细胞毒作用分析

• 批准号: 20790073
• 负责人: 苅谷 由貴子
• 金额: $ 2.58万
• 依托单位: Tohoku Pharmaceutical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790073/
• 关键词: レクチン; Rnase; 癌; 酵素; 糖鎖

Si a lic binding lectin from Rana catesbeiana egges (SBL), which kills various tumor cells, belongs to the RNase A super-family. It has been reported that SBL-induced cell death is closely related to its catalytic activity. However, it still remains to be elucidated how SBL induces cell death by its catalytic activity. To clarify the molecular mechanism of SBL-induced cell death, this study focused on the following things ; (1) cellular localization of SBL, (2) activation of cellular proteins induced by the catalytic activity of SBL, (3) the relationship between the activated proteins and cell death induced by SBL. The results showed that SBL was internalized from early endosomes to a perinuclear region, which was independent on its catalytic activity. Furthermore, SBL catalytic activity induced both stress kinases and caspase-3 activation, and then followed by cell death. These results demonstrate the new molecular mechanism of SBL-induced cell death.

rna结合凝集素（SBL）属于rna酶a超家族，具有杀伤多种肿瘤细胞的作用。有报道称，sbl诱导的细胞死亡与其催化活性密切相关。然而，SBL如何通过其催化活性诱导细胞死亡仍有待阐明。为阐明sbl诱导细胞死亡的分子机制，本研究主要从以下几个方面进行研究；(1) SBL的细胞定位，(2)SBL催化活性诱导的细胞蛋白活化，(3)活化蛋白与SBL诱导的细胞死亡的关系。结果表明，SBL从早期核内体内化到核周区域，与催化活性无关。此外，SBL的催化活性诱导应激激酶和caspase-3激活，然后导致细胞死亡。这些结果揭示了sbl诱导细胞死亡的新的分子机制。

项目成果

シアル酸結合性レクチンは細胞内RNA分解とカスパーゼの活性化を介して細胞死を誘導する

唾液酸结合凝集素通过细胞内 RNA 降解和 caspase 激活诱导细胞死亡

• 发表时间: 2009
• 作者: Yoshiaki Okada;Nobuaki Funahashi;Yoshihiro Kano;Hiroki Naruse;Zhixia Jiang;Enjing Jin;kiichiro Yano;William Aird;Takefumi Doi;河野孝夫;佐賀展子;馬場敦;Atsushi Baba;Atsushi Baba;河野孝夫;馬場敦;石井萌;内田貴之;服部光治;日比輝正;苅谷由貴子
• 通讯作者: 苅谷由貴子