functional analyses of the intracellular phospholipase A1 family in testes

A1 （PLA1）的磷脂酶从二酰基甘油磷脂中切割sn-1脂肪酸酰基，产生游离脂肪酸和sn-2溶血磷脂。细胞内磷脂酶A1（2009年命名为iPLA1）家族由三个成员组成，α,β,γ。为了揭示iPLA1家族成员的生理功能，通过基因靶向产生iPLA1 -β，-γ敲除小鼠（KO）。iPLA1-β和-γKO小鼠出生，与野生型小鼠明显无法区分。然而，雄性iPLA1-β KO小鼠是低生育能力的。

Phospholipases of the A1 (PLA1) cleave the sn-1 fatty acyl group from diacyl glycero-phospholipids and produce a free fatty acid and an sn-2-lysophospholipid. The intracellular phospholipase A1 (named iPLA1 from 2009 ) family consists of three members,α,β,γ. To reveal the physiological functions of iPLA1 family member, iPLA1 -β,-γknockout mice(KO)were generated by gene targeting. iPLA1-β and -γKO mice were born and were apparently indistinguishable from wild-type mice. However, male iPLA1-β KO mice were subfertile.