The effect of ethyl tertiary-butyl ether, a bioethanol-derived fuel oxygenate, on immune responses

乙基叔丁基醚（一种生物乙醇衍生的燃料含氧化合物）对免疫反应的影响

• 批准号: 20790122
• 负责人: YAMAKI Kouya
• 金额: $ 2.66万
• 依托单位: Kobe Pharmaceutical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790122/
• 关键词: ETBE; バイオエタノール; 免疫; T細胞

To determine the effect of ETBE, a bioethanol-derived fuel oxygenate, on immune response, the adjuvant effect of ETBE was investigated. First, we injected ovalbumin and 0.1 to 3 % ETBE in saline solution to mice. After 21 days, anti-OVA IgG levels in serum of the mice were measured. The mice injected with 3 % ETBE were dead within a day. ETBE at concentrations between 0.1 to 1 % did not affect anti-OVA IgG level, thus single injection of ETBE with the antigen was considered to have no adjuvant effect on the antibody production. Next, we injected 1 % ETBE 5 times per week from day 0 to day 20 to mice immunized with ovalbumin on day 0. The levels of anti-OVA IgG in serum of immunized mice with successive injection of 1 % ETBE were higher than those of immunized mice without injection of ETBE. This result indicated that the chronic exposure of ETBE might exert adjuvant activity.In addition, 1 % ETBE treatment attenuated the degranulation of mast cell line RBL2H3.These results indicated 1 % ETBE may potentially affect immune responses. Although low concentrations of ETBE evaporated from fuels might not affect human health, accidental exposure to a high concentration of ETBE is likely to affect immune responses.

为了确定ETBE（一种生物乙醇衍生的燃料添加剂）对免疫应答的作用，研究了ETBE的佐剂作用。首先，我们将卵清蛋白和0.1 - 3%ETBE的盐水溶液注射到小鼠体内。21天后，测量小鼠血清中的抗OVA IgG水平。注射3%ETBE的小鼠在一天内死亡。浓度为0.1 - 1%的ETBE不影响抗OVA IgG水平，因此认为单次注射ETBE和抗原对抗体产生没有佐剂作用。接下来，我们从第0天至第20天每周5次向在第0天用卵清蛋白免疫的小鼠注射1%ETBE。连续注射1%ETBE免疫小鼠血清中抗OVAIgG水平高于未注射ETBE免疫小鼠。结果表明，长期暴露ETBE可能具有一定的免疫佐剂活性，1%ETBE可明显抑制肥大细胞系RBL 2 H3的脱颗粒，提示1%ETBE可能影响机体的免疫功能。虽然从燃料中蒸发的低浓度ETBE可能不会影响人体健康，但意外暴露于高浓度ETBE可能会影响免疫反应。

项目成果

ANTIOXIDATIVE ROLE OF INTERLEUKIN-6 IN SEPTIC LUNG INJURY IN MICE

• DOI: 10.1177/039463200802100303
• 发表时间: 2008-07-01
• 期刊: INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY
• 影响因子: 3.5
• 作者: Inoue, K.;Takano, H.;Yoshikawa, T.
• 通讯作者: Yoshikawa, T.

Inhibition of IgE‐induced mast cell activation by ethyl tertiary‐butyl ether, a bioethanol‐derived fuel oxygenate

乙基叔丁基醚（一种生物乙醇衍生的燃料含氧化合物）抑制 IgE 诱导的肥大细胞活化

• DOI: 10.1211/jpp.61.09.0015
• 发表时间: 2009
• 期刊: Journal of Pharmacy and Pharmacology
• 影响因子: 3.3
• 作者: K. Yamaki;S. Yoshino
• 通讯作者: S. Yoshino

抗卵白アルブミンIgG3モノクローナル抗体の作製およびそれらの in vivo、in vitro における生理活性の解析

抗卵清蛋白IgG3单克隆抗体的制备及其体内外生理活性分析

• 发表时间: 2010
• 作者: 八巻耕也;日高総太;門前縁;吉野伸
• 通讯作者: 吉野伸

I型アレルギー反応のブチルヒドロキシトルエン(BHT)による増強

丁基羟基甲苯 (BHT) 增强 I 型过敏反应

• 发表时间: 2008
• 作者: 八巻耕也;吉野伸
• 通讯作者: 吉野伸

Zinc Oxideナノ粒子がラット好塩基球性白血病細胞株RBL2H3の活性化に与える影響

纳米氧化锌对大鼠嗜碱性白血病细胞系RBL2H3活化的影响

• 发表时间: 2009
• 作者: 八巻耕也;吉野伸
• 通讯作者: 吉野伸