An orally applicable Shiga toxin neutralizer functions in the intestine to inhibit the intracellular transport of the toxin.

我们发现，PPP-tet，一种基于Stx2的多肽抑制剂，完全抑制了直接注射Stx2在兔回肠内造成的液体积聚。我们还发现，PPP-tet仅通过与Stx2形成复合体在回肠上皮细胞中积聚。在培养的上皮细胞中，该复合体阻止了Stx2从高尔基体到内质网的细胞内运输，这一过程是Stx2细胞毒性所必需的。我们的结果表明，PPP-tet通过改变上皮细胞中Stx2的细胞内转运，在肠道中起到了强大的STX中和剂的作用。

We found that PPP-tet, a peptide-based Stx2 inhibitor, completely inhibited fluid accumulation in the rabbit ileum caused by the direct injection of Stx2. We also found that PPP-tet accumulated in the ileal epithelial cells only through its formation of a complex with Stx2. The complex in cultured epithelial cells blocked the intracellular transport of Stx2 from the Golgi apparatus to the endoplasmic reticulum, the process that is essential for Stx2 cytotoxicity. Our results reveal that PPP-tet functions as a potent Stx neutralizer in the intestine by altering the intracellular transport of Stx2 in epithelial cells.