Control of transgenes with insulators about malaria parasite

转基因方法的发展作为疟疾研究中的一种分析技术正在取得进展。当将人工构建的转基因载体引入寄生虫时，基因表达有时不会在预期的时间点发生。然而，已知位于基因组DNA上相邻基因之间的称为“绝缘子”的区域，使用附加体基因插入相邻转基因之间，转录干扰在人类细胞中被抑制。为了评估疟原虫绝缘子的活性，并确定疟原虫基因组DNA上潜在的绝缘子，我们开发了一种疟原虫绝缘子检测系统。

The development of transgenic methods is advancing as an analytical technique in malaria research. When an artificially constructed transgene vector is introduced into parasites, gene expression sometimes does not occur at the expected time point. However, a region called the "insulator" is known to be located between neighboring genes on the genome DNA, is inserted between adjacent transgenes using an episomal gene, the transcript interference was suppressed in human cells. To evaluate the activity of insulator in malaria parasite and identify a potential insulator on the genome DNA of malaria parasite, we developed assay system using a malaria parasite.