Molecular mechanism underlying Super Th1 cell induction

在本项目中，我通过研究GATA3转录因子的表达来研究Th1细胞来源的Super Th1细胞产生IL-13的机制。我首先揭示了超级Th1细胞中IL-13的产生是在转录水平上进行调节的。接下来，我证明了在存在IL-4的情况下，通过TCR、IL-2和IL-18的共同刺激在Th1细胞中诱导了GATA3，并且GATA3是激活Super Th1细胞中IL13转录所必需的。此外，芯片实验证实GATA3与IL13基因上的CGRE基序结合，可能诱导了Super Th1细胞表达IL13。

In this project, I studied the mechanism underlying IL-13 production in Th1 cell-derived Super Th1 cell by focusing on the expression of Gata3 transcription factor. I first revealed that IL-13 production in Super Th1 cells is regulated at the level of transcription. I next demonstrated that Gata3 is induced in Th1 cells by costimulation of TCR, IL-2, and IL-18 in the presence of IL-4 and that Gata3 is necessary to activate Il13 transcription in Super Th1 cells. Moreover, ChIP assay verified that Gata3 binds to a CGRE motif on Il13 gene, which might induce Il13 expression in Super Th1 cells.