Elucidate the pathogenic mechanism of Helicobacter pyloriassociated chronic idiopathic thrombocytopenic purpura

我们报道了由细菌17-kDa蛋白、血小板和抗H.幽门螺杆菌抗体参与幽门螺杆菌相关性慢性特发性血小板减少性紫癜(CITP)的发生。幽门螺杆菌外膜蛋白(OMP)被认为是与Hp相关性CITP相关的细菌因子之一。将制备的重组融合蛋白(ROMP)免疫兔，获得多克隆抗体。证实了该抗体的特异性，该抗体可用于检测幽门螺杆菌的单株OMP。OMP被鉴定为幽门螺杆菌的17 kDa蛋白之一，它与血小板结合，导致聚集。

We reported the possibility that the immunocomplex consisting of bacterial 17-kDa protein, platelet and anti-H. pylori antibodies were involved in development of H. pylori-associated chronic idiopathic thrombocytopenic purpura (cITP). The H. pylori outer membrane protein (OMP) was identified among 11 candidates as one of bacterial factors involved in development of H. pylori - associated cITP. The recombinant fusion protein (rOMP) prepared was immunized with a rabbit to obtain polyclonal antibody. The specificity of the antibody was confirmed and this antibody could detect the OMP among individual H. pylori strains. The OMP was identified as one of the 17-kDa proteins of H. pylori, which binds to platelet, leading to aggregation.