Clarification of urate transporter molecular complex and dynamic regulation in the kidney.

肾脏尿酸盐转运系统在血浆尿酸盐水平的调节中起重要作用。URAT 1是肾脏中主要的尿酸盐转运蛋白之一。因此，本研究的重点是阐明URAT 1相互作用蛋白及其通过磷酸化的动态调节。URAT 1被证明是结合到一个有机阴离子转运蛋白，OAT 4，通过支架蛋白，PDZK 1。此外，当每个转运蛋白与PDZK 1相互作用时，观察到转运活性增加。与PDZK 1的URAT 1结合位点中的磷酸化模拟突变消除URAT 1-PDZK 1相互作用的事实一起，提出了含有“尿酸盐转运体”的URAT 1的新调节机制。

Renal urate transport system plays an important role in the regulation of plasma urate level. URAT1 is one of the major urate transporters in the kidney. Therefore, this study focused on elucidating URAT1-interacting protein(s) and their dynamic regulation through phosphorylation. URAT1 was shown to be bound to an organic anion transporter, OAT4, via a scaffold protein, PDZK1. In addition, increased transport activity was observed when each transporter interacted with PDZK1. Together with the fact that a phosphomimetic mutation in the URAT1 binding site of PDZK1 abolished the URAT1-PDZK1 interaction suggest a novel regulation mechanism for a URAT1 containing "urate transportsome".