Elucidation of urate transport mechanism of a novel urate transporter NPT4 by PDZ Domain Containing 1(PDZK1)

通过酵母双杂交和免疫沉淀实验，我们发现多价pdz结构域蛋白PDZK1与NPT4相互作用。PDZK1与NPT4的相互作用需要NPT4的c端PDZ基序和PDZK1的PDZ域2。此外，通过蛋白质组学方法发现NPT4和PDZK1都与MDR1相互作用。MDR1属于ABC转运蛋白家族，负责转运蛋白。提示NPT4、PDZK1和MDR1形成一个复合物，该复合物参与了尿酸转运。

We found that multivalent PDZ-domain protein PDZK1 interacted with NPT4 by Yeast two hybrid assay and immunoprecipitation. The interaction of PDZK1 with NPT4 requires C-terminal PDZ motif of NPT4 and PDZ domain2 of PDZK1. In addition, it is found by proteomic approach that both NPT4 and PDZK1 interact with MDR1. MDR1 belongs to ABC transporterfamily, and MDR1 transports urate. It suggests that NPT4, PDZK1 and MDR1 to form a complex, and the complex is involved in urate transport.