The effect of B cell depletion on humoral responses during anit-CD20 mAb immunotherapy

anti-CD20 mAb 免疫治疗期间 B 细胞耗竭对体液反应的影响

• 批准号: 20790790
• 负责人: HAMAGUCHI Yasuhito
• 金额: $ 2.75万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20790790/
• 关键词: B細胞; CD20; 免疫応答

The full effects of B cell depletion on humoral immunity are largely unknown. To address this issue, mature B cells were depleted in mice using CD20 mAb. CD20^+ B cell depletion prevented humoral immune responses and class switching, and depleted B cell memory. Nonetheless, B cell depletion did not affect serum Ig levels, Ag-specific antibody titers, or bone marrow antibody-secreting plasma cell numbers. Thus, depleting mature and memory B cells does not have a dramatic negative effect on pre-existing antibody levels.

B细胞耗竭对体液免疫的全部影响在很大程度上是未知的。为了解决这个问题，使用CD20 mAb在小鼠中耗尽成熟B细胞。CD20^+ B细胞耗竭阻止体液免疫应答和类别转换，并耗竭B细胞记忆。尽管如此，B细胞耗竭并不影响血清IG水平、Ag特异性抗体滴度或骨髓抗体分泌浆细胞数量。因此，耗尽成熟和记忆B细胞对预先存在的抗体水平没有显著的负面影响。