Analysis of miRNA expression and function in prostate cancer cells using bioinformatics

在一些癌细胞中，微小RNA(MiRNA)被认为与生长和侵袭有关。然而，miRNA的功能仍不清楚。我们研究了miRNA在前列腺癌中的表达及其功能。我们使用miRNA数据库和前列腺癌中的基因组异常位点来筛选靶miRNA。此外，我们利用定量聚合酶链式反应检测了miRNA在前列腺癌组织和前列腺癌细胞系中的表达。结果，我们发现了miR-30d。MiR-30d抑制剂可抑制前列腺癌细胞株的生长。因此，这些数据表明miR-30d对前列腺癌细胞的生长有重要作用。

Micro RNA (miRNA) is considered the responsibility of growth and invasion in some cancer cells. However, the function of miRNA is still unclear. We investigated miRNA expression and the function in prostate cancer. We used the databases of miRNA and the genome abnormality sites in prostate cancer for the screening of the target miRNA. Further, we examined the expression of miRNA in prostate cancer tissues and prostate cancer cell lines using quantitative PCR. As the result, we found miR-30d. The inhibitor for miR-30d was reduced the growth of prostate cancer cell lines. Thus, these data was suggested that miR-30d had an important role for prostate cancer cell growth.