Investigation to determine the cause of interstitial pneumonia by the EGFR inhibitors, and the development of that suppression method

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Acute interstitial pneumonia is one of serious side effects of epidermal growth factor receptor (EGFR) inhibitor, although that cause is still unknown. Here we report that cancer cells treated with EGFR inhibitors induce fibrosis of normal lung cell. Human tangue squamous cell carcinoma (HSC-3) was cultured with EGFR-tyrosine kinase inhibitor (AG1478) or EGFR antibody. IL-6 expression level was elevated by EGFR inhibitors in HSC-3. Those supernatants were used as the conditioned medium. Then, OUS-11, normal human lung fibroblast-like cell line, was cultured with the conditioned medium. We investigated the expression of collagen, which was the marker for fibrosis, to confirm whether fibrosis was induced or not in OUS-11. That result showed that the expression level of collagen in OUS-11 treated with conditioned medium was higher than control cells. These data suggested that acute interstitial pneumonia was induced IL-6 from cancer cells treated with EGFR inhibitors.