Localization and quantitative analysis of retinal protein by lasermicrodissection

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To study the change of antioxidants in retinal layers, we used lasemicrodissection. Light damaged rat retina were divided into 4 segments ; retinal pigment epithelium, rod outer segments～outer plexiform layer, inner nuclear layer～inner plexiform layer, ganglion cell layer～nerve fiber layer. Antioxidants were increased in each layer, particularly in rod outer segments～outer plexiform layer, and the change of localization and expression were almost similar to the results by immunohisochemical method. Lasermicrodissection is useful to study the localization and quantitative change of protein at cellular levels.