The role of limbal melanocytes in interaction between limbal stem cells and melanocytes

角膜缘黑素细胞在角膜缘干细胞与黑素细胞相互作用中的作用

• 批准号: 20791276
• 负责人: KAWASHIMA Motoko
• 金额: $ 2.66万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20791276/
• 关键词: 再生医学; 外科; 細胞組織

On the ocular surfaces, limbal epithelial stem cells are maintained in undifferentiated and quiescent state in specialized microenvironment termed stem cell niche. To demonstrate the melanocytes are the one of the niche of limbal stem cells, at first we established the isolation and cultivated methods of limbal melanocytes, and performed co-culture of corneal epithelial cells and melanocytes in various conditions.

在眼表面，角膜缘上皮干细胞在特殊的微环境中保持未分化和静止状态，称为干细胞龛。为了证明黑素细胞是角膜缘干细胞的生态区之一，我们首先建立了角膜缘黑素细胞的分离和培养方法，并在不同条件下进行了角膜上皮细胞与黑素细胞的共培养。