Analysis of cis-reguratory elements and its ability to dedifferentiation of mature cells.

顺式调控元件及其对成熟细胞去分化的能力分析。

• 批准号: 20791461
• 负责人: NAKATA Hidemi
• 金额: $ 2.75万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20791461/
• 关键词: 唇顎口蓋裂; MSX1; Msx1; 脱分化; 再生医療; 遺伝子調節領域; アデノウイルスベクター

To analyze the ability of Msx1 to dedifferentiation in vitro and in vivo, we generated eGFP reported adeno viral vector which carried mouse msh-like1 (a homolog of human Msx1) cDNA and without containing msh-like1 sequences for control vector. After infection of these viral vectors to various mature cells, we extracted mRNA and RT-PCR was performed by RUNX2 for the marker of dedifferentiation and GAPDH for house keeping gene.

为了分析Msx1在体外和体内去分化的能力，我们生成了eGFP报告的腺​​病毒载体，该载体携带小鼠msh-like1（人Msx1的同源物）cDNA并且不包含msh-like1序列作为对照载体。将这些病毒载体感染各种成熟细胞后，我们提取mRNA，并通过RUNX2进行RT-PCR作为去分化标记，通过GAPDH进行管家基因。

项目成果

Anterior tooth replacement with implants inalveolar cleft

牙槽裂内种植体替换前牙

• 发表时间: 2009

Anterior tooth replacement with implants in alveolar cleft sites

在牙槽裂部位用种植体替换前牙

• 发表时间: 2009
• 作者: 杉戸孝行;Hidemi Nakata
• 通讯作者: Hidemi Nakata

Clinical Evaluation on ImplantTreatment for CLP Patients

CLP患者种植治疗的临床评价

• 发表时间: 2008